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Literature summary extracted from

  • Wu, B.; Yu, Q.; Chang, S.; Pedroso, M.M.; Gao, Z.; He, B.; Schenk, G.
    Expansin assisted bio-affinity immobilization of endoxylanase from Bacillus subtilis onto corncob residue Characterization and efficient production of xylooligosaccharides (2019), Food Chem., 282, 101-108 .
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.8 the immobilized enzyme retains more than 70% of its initial capability for xylooligosaccharide production after five reaction cycles Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Bacillus subtilis A0A172MAU1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 xylan + H2O the hydrolysis products are mainly xylobiose (57.5% with immobilized enzyme, 36.1% with soluble enzyme) and xylotriose (38.4% with immobilized enzyme, 48.7% with soluble enzyme) Bacillus subtilis xylobiose + xylotriose + xylotetraose
-
 ?
3.2.1.8 xylooligosaccharides + H2O
-
 Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase LC9
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 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
 immobilized enzyme and soluble enzyme Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 30 70 30°C: immobilized enzyme shows about 50% of maximal activity, soluble enzyme shows about 40% of maximal activity. 70°C: immobilized enzyme shows about 65% of maximal activity, soluble enzyme shows about 50% of maximal activity Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
 3 h, immobilized enzyme retains 45.3% of its activity, soluble enzyme retains 16.3% of its activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
 immobilized enzyme and soluble enzyme Bacillus subtilis

General Information

EC Number General Information Comment Organism
3.2.1.8 additional information a one-step method to immobilize xylanase onto cellulosic material by fusion of expansin from Bacillus subtilis to xylanase LC9 without the requirement of prior purification of enzyme is developed. Fusion enzyme EXLXR2-XYN is specifically adsorbed onto corncob residue with high loading capacity due to bio-affinity adsorption of expansin onto cellulose. The immobilization yield is close to 100%, with a recovered activity of 82.4% Bacillus subtilis