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Literature summary extracted from

  • Li, L.J.; Wu, Z.Y.; Yu, Y.; Zhang, L.J.; Zhu, Y.B.; Ni, H.; Chen, F.
    Development and characterization of an alpha-L-rhamnosidase mutant with improved thermostability and a higher efficiency for debittering orange juice (2018), Food Chem., 245, 1070-1078 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.40 food industry the enzyme can remove the bitter taste of naringin from citrus juices. Improvement of thermostabilty can promote the practical value of the enzyme in citrus juice processing Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expression in Pichia pastoris Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.40 V529A mutant enzyme with improved thermostability, improved substrate affinity and better resistance to the inhibition of glucose Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 8.84
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 60°C, mutant enzyme V529A Aspergillus niger
3.2.1.40 12.9
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 60°C, wild-type enzyme Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Aspergillus niger R9W5L0
-
-
3.2.1.40 Aspergillus niger JMU-TS528 R9W5L0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus niger 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus niger JMU-TS528 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 ginsenoside Rg2 + H2O low activity Aspergillus niger ginsenoside Rh1 + alpha-L-rhamnose
-
 ?
3.2.1.40 ginsenoside Rg2 + H2O low activity Aspergillus niger JMU-TS528 ginsenoside Rh1 + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O
-
 Aspergillus niger hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O
-
 Aspergillus niger JMU-TS528 hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 myricitrin + H2O low activity Aspergillus niger 3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Aspergillus niger beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Aspergillus niger JMU-TS528 beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Aspergillus niger isoquercitrin + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Aspergillus niger JMU-TS528 isoquercitrin + alpha-L-rhamnose
-
 ?
3.2.1.40 saikosaponin C + H2O low activity Aspergillus niger (3beta,16beta)-16-hydroxy-13,28-epoxyolean-11-en-3-yl) 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + alpha-L-rhamnose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 60
-
 T1/2: 14.2 h (mutant enzyme V529A), 12.28 h (wild-type enzyme) Aspergillus niger
3.2.1.40 65
-
 T1/2: 50 min (mutant enzyme V529A), 35 min (wild-type enzyme) Aspergillus niger
3.2.1.40 70
-
 T1/2: 6.35 min (mutant enzyme V529A), 4.35 min (wild-type enzyme) Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.40 5380
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 60°C, mutant enzyme V529A Aspergillus niger
3.2.1.40 10200
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 60°C, wild-type enzyme Aspergillus niger

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.40 608.6
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 60°C, mutant enzyme V529A Aspergillus niger
3.2.1.40 792
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.0, 60°C, wild-type enzyme Aspergillus niger