EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.42 | food industry | the treatment of the wheat dough with the recombinant enzyme causes a softening. The enzyme does not increase the specific bread volume, but improves dough handling. The enzyme may be effective to improve the taste and digestibility of coffee beverages | Ustilago maydis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.42 | expression in Pichia pastoris | Ustilago maydis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.42 | 0.0263 | - |
chlorogenate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 0.1251 | - |
methyl caffeate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 0.1796 | - |
methyl ferulate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 0.2342 | - |
methyl p-coumarate | pH 7.5, 37°C | Ustilago maydis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.42 | Ustilago maydis | A0A060S684 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.42 | glycoprotein | treatment with endoglycosidase H decreases molecular mass from 87000 Da to 63000 Da | Ustilago maydis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.42 | 6.38 | - |
substrate chlorogenate, pH 7.5, 37°C | Ustilago maydis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.42 | alpha-L-galactopyranosyl-(1,2)-beta-D-xylopyranosyl-(1,2)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 27% of the activity with chlorogenate | Ustilago maydis | ? | - |
? | |
3.1.1.42 | beta-D-xylopyranosyl-(1,2)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 54% of the activity with chlorogenate | Ustilago maydis | ? | - |
? | |
3.1.1.42 | chlorogenate + H2O | - |
Ustilago maydis | caffeate + quinate | - |
? | |
3.1.1.42 | D-xylopyranosyl-(1,4)-beta-D-xylopyranosyl-(1,3)-5-O-trans-feruloyl-L-arabinofuranose + H2O | 38% of the activity with chlorogenate | Ustilago maydis | ? | - |
? | |
3.1.1.42 | ethyl ferulate + H2O | 28% of the activity with chlorogenate | Ustilago maydis | ethanol + ferulate | - |
? | |
3.1.1.42 | L-arabinose 5-feruloyl ester + H2O | 63% of the activity with chlorogenate | Ustilago maydis | L-arabinose + ferluate | - |
? | |
3.1.1.42 | methyl caffeate + H2O | 15% of the activity with chlorogenate | Ustilago maydis | methanol + caffeate | - |
? | |
3.1.1.42 | methyl ferulate + H2O | 35% of the activity with chlorogenate | Ustilago maydis | methanol + ferulate | - |
? | |
3.1.1.42 | methyl p-coumarate + H2O | 37% of the activity with chlorogenate | Ustilago maydis | methanol + p-coumarate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.42 | ? | x * 87000, SDS-PAGE, x * 62300, calculated from sequence, His-tagged recombinant protein | Ustilago maydis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.42 | chlE | - |
Ustilago maydis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.42 | 37 | - |
1 h, 20% loss of activity | Ustilago maydis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.42 | 1.9 | - |
methyl caffeate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 7.1 | - |
methyl ferulate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 7.3 | - |
methyl p-coumarate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 19 | - |
chlorogenate | pH 7.5, 37°C | Ustilago maydis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.42 | 7.5 | - |
- |
Ustilago maydis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.42 | 2 | 10 | recombinant enzyme, stable over the entire pH range examined | Ustilago maydis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.42 | 14.8 | - |
methyl caffeate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 31 | - |
methyl p-coumarate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 39.3 | - |
methyl ferulate | pH 7.5, 37°C | Ustilago maydis | |
3.1.1.42 | 723.3 | - |
chlorogenate | pH 7.5, 37°C | Ustilago maydis |