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Literature summary extracted from
- Characterization of an acidic cold-adapted cutinase from Thielavia terrestris and its application in flavor ester synthesis (2015), Food Chem., 188, 439-445 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.74 | synthesis | the cutinase exhibits strong synthetic activity for butyl butanoate in non-aqueous environment,under the optimized reaction conditions esterification efficiency of 95% is observed | Thermothielavioides terrestris |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.74 | 0.88 | - |
4-nitrophenyl caproate | pH 4.0, 50°C | Thermothielavioides terrestris |  |
| 3.1.1.74 | 1.1 | - |
4-nitrophenyl butanoate | pH 4.0, 50°C | Thermothielavioides terrestris | |
| 3.1.1.74 | 8.3 | - |
4-nitrophenyl acetate | pH 4.0, 50°C | Thermothielavioides terrestris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.74 | 27900 | - |
gel filtration | Thermothielavioides terrestris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.74 | Thermothielavioides terrestris | - |
- |
- |
| 3.1.1.74 | Thermothielavioides terrestris CAU709 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.74 | - |
Thermothielavioides terrestris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.74 | 983 | - |
substrate 4-nitrophenyl butanoate, pH 4.0, 50°C | Thermothielavioides terrestris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.74 | 4-nitrophenyl acetate + H2O | - |
Thermothielavioides terrestris | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.74 | 4-nitrophenyl acetate + H2O | - |
Thermothielavioides terrestris CAU709 | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.74 | 4-nitrophenyl butanoate + H2O | - |
Thermothielavioides terrestris | 4-nitrophenol + butanoate | - |
? | |
| 3.1.1.74 | 4-nitrophenyl butanoate + H2O | - |
Thermothielavioides terrestris CAU709 | 4-nitrophenol + butanoate | - |
? | |
| 3.1.1.74 | 4-nitrophenyl caproate + H2O | - |
Thermothielavioides terrestris | 4-nitrophenol + caproate | - |
? | |
| 3.1.1.74 | 4-nitrophenyl caproate + H2O | - |
Thermothielavioides terrestris CAU709 | 4-nitrophenol + caproate | - |
? | |
| 3.1.1.74 | butanol + butanoate | - |
Thermothielavioides terrestris | butyl butanoate + H2O | - |
? | |
| 3.1.1.74 | tributyrin + H2O | - |
Thermothielavioides terrestris | ? | - |
? | |
| 3.1.1.74 | tributyrin + H2O | - |
Thermothielavioides terrestris CAU709 | ? | - |
? | |
| 3.1.1.74 | tricaproin + H2O | - |
Thermothielavioides terrestris | ? | - |
? | |
| 3.1.1.74 | tricaproin + H2O | - |
Thermothielavioides terrestris CAU709 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.74 | monomer | 1 * 27300, SDS-PAGE | Thermothielavioides terrestris |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.74 | CutB | - |
Thermothielavioides terrestris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.74 | 55 | - |
- |
Thermothielavioides terrestris |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.74 | - |
- |
more than 30% residual activity | Thermothielavioides terrestris |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.74 | 65 | - |
stable up to | Thermothielavioides terrestris |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.74 | 0.58 | - |
4-nitrophenyl acetate | pH 4.0, 50°C | Thermothielavioides terrestris | |
| 3.1.1.74 | 0.61 | - |
4-nitrophenyl caproate | pH 4.0, 50°C | Thermothielavioides terrestris | |
| 3.1.1.74 | 0.85 | - |
4-nitrophenyl butanoate | pH 4.0, 50°C | Thermothielavioides terrestris | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.74 | 4 | - |
- |
Thermothielavioides terrestris |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.74 | 2.5 | 10.5 | - |
Thermothielavioides terrestris |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.74 | 0.07 | - |
4-nitrophenyl acetate | pH 4.0, 50°C | Thermothielavioides terrestris | |
| 3.1.1.74 | 0.68 | - |
4-nitrophenyl caproate | pH 4.0, 50°C | Thermothielavioides terrestris | |
| 3.1.1.74 | 0.77 | - |
4-nitrophenyl butanoate | pH 4.0, 50°C | Thermothielavioides terrestris | |
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