EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.17 | expressed in Escherichia coli Rosetta (DE3) cells | Apostichopus japonicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.17 | Apostichopus japonicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.17 | Ni-NTA Sepharose column chromatography | Apostichopus japonicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.17 | body wall | - |
Apostichopus japonicus | - |
3.2.1.17 | coelomocyte | - |
Apostichopus japonicus | - |
3.2.1.17 | intestine | lowest expression | Apostichopus japonicus | - |
3.2.1.17 | respiratory system | - |
Apostichopus japonicus | - |
3.2.1.17 | tentacle | highest expression | Apostichopus japonicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.17 | cell wall of Micrococcus lysodeikticus + H2O | - |
Apostichopus japonicus | ? | - |
? | |
3.2.1.17 | additional information | the enzyme also has isopeptidase activity with 4-nitroanilide-L-gamma-glutamic acid | Apostichopus japonicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.17 | ? | x * 14300, calculated from amino acid sequence | Apostichopus japonicus |
3.2.1.17 | ? | x * 32200, Thioredoxin-His-S-tagged enzyme, SDS-PAGE | Apostichopus japonicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.17 | iLys | - |
Apostichopus japonicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.17 | 37 | - |
optimum temperature for isopeptidase activity | Apostichopus japonicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.17 | 6.5 | - |
optimum pH for isopeptidase activity | Apostichopus japonicus |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.2.1.17 | Apostichopus japonicus | challenge with the pathogen Vibrio splendidus and lipopolysaccharide stimulation both significantly up-regulates the mRNA expression of the enzyme | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.17 | physiological function | the enzyme serves as an important innate immunity factor and plays a key defense role during host-pathogen interactions in sea cucumbers | Apostichopus japonicus |