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Literature summary extracted from
- Characterization and in vitro sensitivity of cholinesterases of gilthead seabream (Sparus aurata) to organophosphate pesticides (2017), Fish Physiol. Biochem., 43, 455-464 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.7 | 1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide | i.e. BW284c51, shows inhibition at high substrate concentrations | Sparus aurata |  |
| 3.1.1.7 | azinphosmethyl-oxon | - |
Sparus aurata | |
| 3.1.1.7 | dichlorvos | - |
Sparus aurata | |
| 3.1.1.7 | additional information | in vitro sensitivity of cholinesterases from gilthead seabream (Sparus aurata) to organophosphate pesticides (OPs), overview. The inhibitory potency of OPs on brain and muscle AChEs based on bimolecular inhibition constants (ki) is in ascending order: omethoate, dichlorvos, azinphosmethyl-oxon | Sparus aurata | |
| 3.1.1.7 | omethoate | - |
Sparus aurata | |
| 3.1.1.8 | azinphosmethyl-oxon | - |
Sparus aurata | |
| 3.1.1.8 | dichlorvos | - |
Sparus aurata | |
| 3.1.1.8 | additional information | in vitro sensitivity of cholinesterases from gilthead seabream (Sparus aurata) to organophosphate pesticides, overview. Muscle BChE is found to be several orders of magnitude (from 2 to 4) more sensitive than brain and muscle acetylcholinesterase (AChE, EC 3.1.1.7) inhibition by dichlorvos and omethoate. Enzyme BChE does not show excess substrate inhibition | Sparus aurata | |
| 3.1.1.8 | omethoate | - |
Sparus aurata | |
| 3.1.1.8 | tetraisopropylpyrophosphoramide | iso-OMPA, enzyme BChE is selectively and completely inhibited | Sparus aurata | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.7 | additional information | - |
additional information | Michaelis-Menten kinetics | Sparus aurata | |
| 3.1.1.7 | 0.16 | - |
acetylthiocholine | pH 7.4, 25°C, enzyme from brain | Sparus aurata | |
| 3.1.1.7 | 0.24 | - |
acetylthiocholine | pH 7.4, 25°C, enzyme from muscle | Sparus aurata | |
| 3.1.1.8 | additional information | - |
additional information | Michaelis-Menten kinetics | Sparus aurata | |
| 3.1.1.8 | 0.16 | - |
butyrylthiocholine | pH 7.4, 25°C, enzyme from muscle | Sparus aurata | |
| 3.1.1.8 | 0.163 | - |
butyrylthiocholine | pH 7.4, 25°C, enzyme from yolk-sac of seabream larvae stage | Sparus aurata | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.7 | acetylcholine + H2O | Sparus aurata | - |
choline + acetate | - |
? | |
| 3.1.1.8 | butyrylcholine + H2O | Sparus aurata | - |
choline + butyrate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.7 | Sparus aurata | - |
- |
- |
| 3.1.1.8 | Sparus aurata | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.7 | brain | - |
Sparus aurata | - |
| 3.1.1.7 | additional information | AChE is the enzyme present in the brain, whereas in muscle, a typical AChE form is present along with an atypical form of butyrylcholinesterase (BChE, EC 3.1.1.8) | Sparus aurata | - |
| 3.1.1.7 | muscle | - |
Sparus aurata | - |
| 3.1.1.7 | plasma cell | low enzyme level | Sparus aurata | - |
| 3.1.1.8 | brain | low enzyme level | Sparus aurata | - |
| 3.1.1.8 | additional information | acetylcholinesterase (AChE, EC 3.1.1.7) is the enzyme present in the brain, whereas in muscle, a typical formof AChE is present along with an atypical form of butyrylcholinesterase (BChE) | Sparus aurata | - |
| 3.1.1.8 | muscle | - |
Sparus aurata | - |
| 3.1.1.8 | plasma cell | low enzyme level | Sparus aurata | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.7 | acetylcholine + H2O | - |
Sparus aurata | choline + acetate | - |
? | |
| 3.1.1.7 | acetylthiocholine + H2O | i.e. acetyl-beta-(methyl)thiocholine iodide (AbMSCh) as substrate | Sparus aurata | thiocholine + acetate | - |
? | |
| 3.1.1.7 | additional information | no activity with butyrylthiocholine | Sparus aurata | ? | - |
? | |
| 3.1.1.8 | acetylthiocholine + H2O | enzyme BChE may hydrolyze in some extent the substrate acetylthiocholine, ASCh | Sparus aurata | thiocholine + acetate | - |
? | |
| 3.1.1.8 | butyrylcholine + H2O | - |
Sparus aurata | choline + butyrate | - |
? | |
| 3.1.1.8 | butyrylthiocholine + H2O | - |
Sparus aurata | thiocholine + butyrate | - |
? | |
| 3.1.1.8 | additional information | BChE hydrolyzes a wider range of esters but preferentially S-butyrylthiocholine iodide (BSCh) | Sparus aurata | ? | - |
? | |
| 3.1.1.8 | propionylthiocholine + H2O | - |
Sparus aurata | thiocholine + propionate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.7 | AChE | - |
Sparus aurata |
| 3.1.1.8 | BChE | - |
Sparus aurata |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.7 | 25 | - |
assay at | Sparus aurata |
| 3.1.1.8 | 25 | - |
assay at | Sparus aurata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.7 | 7.4 | - |
assay at | Sparus aurata |
| 3.1.1.8 | 7.4 | - |
assay at | Sparus aurata |
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