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Literature summary extracted from

  • Shastry, R.P.; Dolan, S.K.; Abdelhamid, Y.; Vittal, R.R.; Welch, M.
    Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66 (2018), FEMS Microbiol. Lett., 365, fny054.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 His6-tagged AiiE is overexpressed from pET-19m in Rosetta DE3 cells Enterobacter sp. CS66

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.81 6.01
-
 N-butanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 7.88
-
 N-hexanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 10.35
-
 N-octanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 14.88
-
 N-decanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 15.21
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 29000
-
 SDS-PAGE Enterobacter sp. CS66

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Enterobacter sp. CS66
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81
-
 Enterobacter sp. CS66

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-butanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-butanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-octanoyl-L-homoserine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.81 ? x * 29000, SDS-PAGE Enterobacter sp. CS66

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 aiiE
-
 Enterobacter sp. CS66
3.1.1.81 quorum quenching AHL-lactonase
-
 Enterobacter sp. CS66

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.81 61.14
-
 N-butanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 69.43
-
 N-hexanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 80.07
-
 N-octanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 98.31
-
 N-decanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 101.5
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.81 6.61
-
 N-decanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 6.67
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 7.74
-
 N-octanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 8.81
-
 N-hexanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
3.1.1.81 10.17
-
 N-butanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66