EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.15 | expressed in Escherichia coli Rosetta-gami 2 cells | Chondrostereum purpureum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.15 | A365P | the mutant shows about 70% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | D395N | the mutant shows about 10% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | E364Q | the mutant shows about 20% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | E364Q/E366Q | the mutant shows about 60% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | H150A | the mutant shows about 20% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | H195A | inactive | Chondrostereum purpureum |
3.2.1.15 | H98A | inactive | Chondrostereum purpureum |
3.2.1.15 | K205A | inactive | Chondrostereum purpureum |
3.2.1.15 | K228A | inactive | Chondrostereum purpureum |
3.2.1.15 | K253A | the mutant shows about 50% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | K318A | inactive | Chondrostereum purpureum |
3.2.1.15 | K88A | the mutant shows about 1% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | P339A | the mutant shows about 5% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | P348A | the mutant shows about 80% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | P352A | the mutant shows about 35% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | P355A | the mutant shows about 30% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | P358A | the mutant shows about 7% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | R220A | the mutant shows about 10% of wild type activity | Chondrostereum purpureum |
3.2.1.15 | R226A | inactive | Chondrostereum purpureum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.15 | Chondrostereum purpureum | P79074 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.15 | Ni-NTA column chromatography and DE52-cellulose column chromatography | Chondrostereum purpureum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 2.33 | - |
crude extract, at pH 4.5 and 30°C | Chondrostereum purpureum |
3.2.1.15 | 435 | - |
after 187fold purification, at pH 4.5 and 30°C | Chondrostereum purpureum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.15 | pectin + H2O | - |
Chondrostereum purpureum | ? | - |
? | |
3.2.1.15 | polygalacturonate + H2O | - |
Chondrostereum purpureum | oligogalacturonates | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.15 | ? | x * 45000, SDS-PAGE | Chondrostereum purpureum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.15 | EndoPG I | - |
Chondrostereum purpureum |
3.2.1.15 | endopolygalacturonase I | - |
Chondrostereum purpureum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 60 | - |
- |
Chondrostereum purpureum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 40 | 80 | about 35% activity at 40°C, about 75% activity at 50°C, 100% activity at 60°C, about 60% activity at 70°C, about 45% activity at 80°C | Chondrostereum purpureum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 30 | 60 | the enzyme activity remains stable (more than 90% activity) after 30 min between 30 and 60°C | Chondrostereum purpureum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.15 | 12.4 | - |
Pectin | pro-enzyme, at pH 4.5 and 30°C | Chondrostereum purpureum | |
3.2.1.15 | 20.2 | - |
Pectin | pro-enzyme, at pH 4.0 and 30°C | Chondrostereum purpureum | |
3.2.1.15 | 230 | - |
Pectin | mature enzyme, at pH 4.5 and 30°C | Chondrostereum purpureum | |
3.2.1.15 | 334 | - |
Polygalacturonate | pro-enzyme, at pH 4.5 and 30°C | Chondrostereum purpureum | |
3.2.1.15 | 996 | - |
Polygalacturonate | mature enzyme, at pH 4.5 and 30°C | Chondrostereum purpureum | |
3.2.1.15 | 1670 | - |
Polygalacturonate | pro-enzyme, at pH 4.0 and 30°C | Chondrostereum purpureum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 4 | - |
- |
Chondrostereum purpureum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 3 | 7 | the enzyme activity remains stable after 16 h between pH 3.0 and 7.0 | Chondrostereum purpureum |