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Literature summary extracted from

  • Hamada, S.; Toda, K.; Ogawa, S.; Kubota, K.; Miyairi, K.
    Characterization of the effects of C-terminal pro-sequence on self-inactivation of Stereum purpureum endopolygalacturonase I (2015), FEMS Microbiol. Lett., 362, fnv134 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.15 expressed in Escherichia coli Rosetta-gami 2 cells Chondrostereum purpureum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.15 A365P the mutant shows about 70% of wild type activity Chondrostereum purpureum
3.2.1.15 D395N the mutant shows about 10% of wild type activity Chondrostereum purpureum
3.2.1.15 E364Q the mutant shows about 20% of wild type activity Chondrostereum purpureum
3.2.1.15 E364Q/E366Q the mutant shows about 60% of wild type activity Chondrostereum purpureum
3.2.1.15 H150A the mutant shows about 20% of wild type activity Chondrostereum purpureum
3.2.1.15 H195A inactive Chondrostereum purpureum
3.2.1.15 H98A inactive Chondrostereum purpureum
3.2.1.15 K205A inactive Chondrostereum purpureum
3.2.1.15 K228A inactive Chondrostereum purpureum
3.2.1.15 K253A the mutant shows about 50% of wild type activity Chondrostereum purpureum
3.2.1.15 K318A inactive Chondrostereum purpureum
3.2.1.15 K88A the mutant shows about 1% of wild type activity Chondrostereum purpureum
3.2.1.15 P339A the mutant shows about 5% of wild type activity Chondrostereum purpureum
3.2.1.15 P348A the mutant shows about 80% of wild type activity Chondrostereum purpureum
3.2.1.15 P352A the mutant shows about 35% of wild type activity Chondrostereum purpureum
3.2.1.15 P355A the mutant shows about 30% of wild type activity Chondrostereum purpureum
3.2.1.15 P358A the mutant shows about 7% of wild type activity Chondrostereum purpureum
3.2.1.15 R220A the mutant shows about 10% of wild type activity Chondrostereum purpureum
3.2.1.15 R226A inactive Chondrostereum purpureum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Chondrostereum purpureum P79074
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 Ni-NTA column chromatography and DE52-cellulose column chromatography Chondrostereum purpureum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 2.33
-
crude extract, at pH 4.5 and 30°C Chondrostereum purpureum
3.2.1.15 435
-
after 187fold purification, at pH 4.5 and 30°C Chondrostereum purpureum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 pectin + H2O
-
Chondrostereum purpureum ?
-
?
3.2.1.15 polygalacturonate + H2O
-
Chondrostereum purpureum oligogalacturonates
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 45000, SDS-PAGE Chondrostereum purpureum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 EndoPG I
-
Chondrostereum purpureum
3.2.1.15 endopolygalacturonase I
-
Chondrostereum purpureum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 60
-
-
Chondrostereum purpureum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 40 80 about 35% activity at 40°C, about 75% activity at 50°C, 100% activity at 60°C, about 60% activity at 70°C, about 45% activity at 80°C Chondrostereum purpureum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 30 60 the enzyme activity remains stable (more than 90% activity) after 30 min between 30 and 60°C Chondrostereum purpureum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.15 12.4
-
Pectin pro-enzyme, at pH 4.5 and 30°C Chondrostereum purpureum
3.2.1.15 20.2
-
Pectin pro-enzyme, at pH 4.0 and 30°C Chondrostereum purpureum
3.2.1.15 230
-
Pectin mature enzyme, at pH 4.5 and 30°C Chondrostereum purpureum
3.2.1.15 334
-
Polygalacturonate pro-enzyme, at pH 4.5 and 30°C Chondrostereum purpureum
3.2.1.15 996
-
Polygalacturonate mature enzyme, at pH 4.5 and 30°C Chondrostereum purpureum
3.2.1.15 1670
-
Polygalacturonate pro-enzyme, at pH 4.0 and 30°C Chondrostereum purpureum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 4
-
-
Chondrostereum purpureum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 3 7 the enzyme activity remains stable after 16 h between pH 3.0 and 7.0 Chondrostereum purpureum