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Literature summary extracted from

  • Oh, C.; Ryu, B.H.; An, D.R.; Nguyen, D.D.; Yoo, W.; Kim, T.; Ngo, T.D.; Kim, H.S.; Kim, K.K.; Kim, T.D.
    Structural and biochemical characterization of an octameric carbohydrate acetylesterase from Sinorhizobium meliloti (2016), FEBS Lett., 590, 1242-1252 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.6 expressed in Escherichia coli BL21(DE3) cells Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.6 using 0.2 M ammonium phosphate (pH 4.6), and 20% (w/v) polyethylene glycol 3350 Sinorhizobium meliloti

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.6 F152A the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis Sinorhizobium meliloti
3.1.1.6 M155A the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis Sinorhizobium meliloti
3.1.1.6 N96A the mutant has almost no activity Sinorhizobium meliloti
3.1.1.6 R69A the mutant has about 25% activity for 1-naphthyl acetate hydrolysis compared to the wild type enzyme Sinorhizobium meliloti
3.1.1.6 S13A the mutant shows complete loss of activity Sinorhizobium meliloti
3.1.1.6 W151A the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis Sinorhizobium meliloti
3.1.1.6 Y101A the mutant has almost no activity Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Sinorhizobium meliloti Q92XZ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6
-
 Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 1-naphthyl acetate + H2O highest activity (100%) Sinorhizobium meliloti 1-naphthol + acetate
-
 ?
3.1.1.6 1-naphthyl butyrate + H2O 36% activity compared to 1-naphthyl acetate Sinorhizobium meliloti 1-naphthol + butyrate
-
 ?
3.1.1.6 2-naphthyl acetate + H2O about 40% activity compared to 1-naphthyl acetate Sinorhizobium meliloti 2-naphthol + acetate
-
 ?
3.1.1.6 4-methylumbelliferyl acetate + H2O
-
 Sinorhizobium meliloti 4-methylumbelliferone + acetate
-
 ?
3.1.1.6 acetyl alginate + H2O
-
 Sinorhizobium meliloti alginate + acetate
-
 ?
3.1.1.6 cellulose acetate + H2O
-
 Sinorhizobium meliloti cellulose + acetate
-
 ?
3.1.1.6 D-glucose penta-acetate + H2O
-
 Sinorhizobium meliloti ?
-
 ?
3.1.1.6 additional information no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil Sinorhizobium meliloti ?
-
 ?
3.1.1.6 tert-butyl acetate + H2O
-
 Sinorhizobium meliloti 2-methylpropane + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.6 octamer
-
 Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
3.1.1.6 carbohydrate acetylesterase
-
 Sinorhizobium meliloti
3.1.1.6 Est24
-
 Sinorhizobium meliloti