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Literature summary extracted from
- Functional identification of sterol-4alpha-methyl oxidase cDNAs from Arabidopsis thaliana by complementation of a yeast erg25 mutant lacking sterol-4alpha-methyl oxidation (2001), FEBS Lett., 508, 39-43 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.18.10 | expressed in Saccharomyces cerevisiae erg25 mutant | Arabidopsis thaliana |
| 1.14.18.11 | expressed in Saccharomyces cerevisiae erg25 ergosterol auxotroph strain | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.10 | 24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ | Arabidopsis thaliana | overall reaction | 3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O | - |
ir |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.10 | Arabidopsis thaliana | Q8VWZ8 | - |
- |
| 1.14.18.10 | Arabidopsis thaliana | Q9ZW22 | - |
- |
| 1.14.18.11 | Arabidopsis thaliana | Q9ZW22 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.10 | 24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ | overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O | - |
ir | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.10 | SMO1 | isoform | Arabidopsis thaliana |
| 1.14.18.10 | SMO2 | isoform | Arabidopsis thaliana |
| 1.14.18.10 | sterol-4alpha-methyl oxidase | - |
Arabidopsis thaliana |
| 1.14.18.11 | SMO2 | - |
Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.10 | cytochrome b5 | - |
Arabidopsis thaliana | |
| 1.14.18.10 | NADH | required | Arabidopsis thaliana | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.18.11 | metabolism | enzyme expression in a yeast erg25 ergosterol auxotroph, lacking sterol-4alpha-methyl oxidase activity, restores growth and endogenous ergosterol synthesis | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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