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Literature summary extracted from

  • Kumar, P.; Kesari, P.; Kokane, S.; Ghosh, D.K.; Kumar, P.; Sharma, A.K.
    Crystal structures of a putative periplasmic cystine-binding protein from Candidatus Liberibacter asiaticus insights into an adapted mechanism of ligand binding (2019), FEBS J., 286, 3450-3472 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.12 expressed in Escherichia coli Rosetta (DE3) cells Candidatus Liberibacter asiaticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.4.2.12 enzyme complex with L-cystine, L-cysteine, L-arginine and citrate, sitting drop vapor diffusion method, using 0.1 M sodium acetate trihydrate buffer, pH 5.6 containing 2.0 M ammonium sulfate Candidatus Liberibacter asiaticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.4.2.12 ATP + H2O + D-cystine-[cystine-binding protein](side 1) Candidatus Liberibacter asiaticus
-
 ADP + phosphate + D-cystine(side 2) + [cystine-binding protein](side 1)
-
 ?
7.4.2.12 ATP + H2O + D-cystine-[cystine-binding protein](side 1) Candidatus Liberibacter asiaticus psy62
-
 ADP + phosphate + D-cystine(side 2) + [cystine-binding protein](side 1)
-
 ?
7.4.2.12 ATP + H2O + L-cystine-[cystine-binding protein](side 1) Candidatus Liberibacter asiaticus
-
 ADP + phosphate + L-cystine(side 2) + [cystine-binding protein](side 1)
-
 ?
7.4.2.12 ATP + H2O + L-cystine-[cystine-binding protein](side 1) Candidatus Liberibacter asiaticus psy62
-
 ADP + phosphate + L-cystine(side 2) + [cystine-binding protein](side 1)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.12 Candidatus Liberibacter asiaticus C6XGT2
-
-
7.4.2.12 Candidatus Liberibacter asiaticus psy62 C6XGT2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.4.2.12 His-Select nickel affinity column chromatography and Superdex S200 gel filtration Candidatus Liberibacter asiaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.12 ATP + H2O + D-cystine-[cystine-binding protein](side 1)
-
 Candidatus Liberibacter asiaticus ADP + phosphate + D-cystine(side 2) + [cystine-binding protein](side 1)
-
 ?
7.4.2.12 ATP + H2O + D-cystine-[cystine-binding protein](side 1)
-
 Candidatus Liberibacter asiaticus psy62 ADP + phosphate + D-cystine(side 2) + [cystine-binding protein](side 1)
-
 ?
7.4.2.12 ATP + H2O + L-cystine-[cystine-binding protein](side 1)
-
 Candidatus Liberibacter asiaticus ADP + phosphate + L-cystine(side 2) + [cystine-binding protein](side 1)
-
 ?
7.4.2.12 ATP + H2O + L-cystine-[cystine-binding protein](side 1)
-
 Candidatus Liberibacter asiaticus psy62 ADP + phosphate + L-cystine(side 2) + [cystine-binding protein](side 1)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.4.2.12 L-cystine ABC transporter
-
 Candidatus Liberibacter asiaticus
7.4.2.12 TcyA
-
 Candidatus Liberibacter asiaticus