EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.23 | expression in Pichia pastoris | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 1.42 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 1.76 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 2.41 | - |
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 104 | - |
lactose | pH 4.5, 60°C | Aspergillus oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 122000 | - |
SDS-PAGE, recombinant glycosylated enzyme | Aspergillus oryzae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | xyloglucan oligosaccharide + H2O | Aspergillus oryzae | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | galactopyranose + xyloglucan oligosaccharide | - |
? | |
3.2.1.23 | xyloglucan oligosaccharide + H2O | Aspergillus oryzae ATCC 42149 | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | galactopyranose + xyloglucan oligosaccharide | - |
? | |
3.2.1.120 | xyloglucan + H2O | Aspergillus oryzae | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | isoprimeverose + ? | - |
? | |
3.2.1.120 | xyloglucan + H2O | Aspergillus oryzae ATCC 42149 | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | isoprimeverose + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.23 | Aspergillus oryzae | Q2UCU3 | - |
- |
3.2.1.23 | Aspergillus oryzae ATCC 42149 | Q2UCU3 | - |
- |
3.2.1.120 | Aspergillus oryzae | Q2U8V9 | - |
- |
3.2.1.120 | Aspergillus oryzae ATCC 42149 | Q2U8V9 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.23 | glycoprotein | recombinant LacA is N-glycosylated and the protein band is shifted from approximately 122000 Da to 108000 Da by endoglycosidase Hf treatment | Aspergillus oryzae |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.23 | recombinant His6-tagged enzyme | Aspergillus oryzae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 3 | 3.9 | substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
3.2.1.23 | 17.7 | - |
substrate: lactose, pH 4.5, 60°C | Aspergillus oryzae |
3.2.1.23 | 35.8 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
3.2.1.23 | 71 | - |
substrate: beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
3.2.1.23 | 121 | - |
substrate: beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
3.2.1.23 | 299 | - |
substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 4.5, 60°C | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.23 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus oryzae ATCC 42149 | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.23 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose | - |
? | |
3.2.1.23 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae ATCC 42149 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose | - |
? | |
3.2.1.23 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose | - |
? | |
3.2.1.23 | beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose | - |
? | |
3.2.1.23 | beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose | - |
? | |
3.2.1.23 | lactose + H2O | - |
Aspergillus oryzae | D-glucose + D-galactose | - |
? | |
3.2.1.23 | lactose + H2O | - |
Aspergillus oryzae ATCC 42149 | D-glucose + D-galactose | - |
? | |
3.2.1.23 | xyloglucan oligosaccharide + H2O | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | Aspergillus oryzae | galactopyranose + xyloglucan oligosaccharide | - |
? | |
3.2.1.23 | xyloglucan oligosaccharide + H2O | the enzyme cleaves galactopyranosyl residues from xyloglucan oligosaccharides, but not from xyloglucan polysaccharide. An isoprimeverose unit at the nonreducing end of the main chain of xyloglucan oligosaccharides blocks LacA activity | Aspergillus oryzae | galactopyranose + xyloglucan oligosaccharide | - |
? | |
3.2.1.23 | xyloglucan oligosaccharide + H2O | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | Aspergillus oryzae ATCC 42149 | galactopyranose + xyloglucan oligosaccharide | - |
? | |
3.2.1.23 | xyloglucan oligosaccharide + H2O | the enzyme cleaves galactopyranosyl residues from xyloglucan oligosaccharides, but not from xyloglucan polysaccharide. An isoprimeverose unit at the nonreducing end of the main chain of xyloglucan oligosaccharides blocks LacA activity | Aspergillus oryzae ATCC 42149 | galactopyranose + xyloglucan oligosaccharide | - |
? | |
3.2.1.120 | xyloglucan + H2O | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | Aspergillus oryzae | isoprimeverose + ? | - |
? | |
3.2.1.120 | xyloglucan + H2O | isoprimeverose-producing oligoxyloglucan hydrolase requires LacA for the removal of galactopyranosyl residues | Aspergillus oryzae | isoprimeverose + ? | - |
? | |
3.2.1.120 | xyloglucan + H2O | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | Aspergillus oryzae ATCC 42149 | isoprimeverose + ? | - |
? | |
3.2.1.120 | xyloglucan + H2O | isoprimeverose-producing oligoxyloglucan hydrolase requires LacA for the removal of galactopyranosyl residues | Aspergillus oryzae ATCC 42149 | isoprimeverose + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.23 | LacA | - |
Aspergillus oryzae |
3.2.1.120 | IpeA | - |
Aspergillus oryzae |
3.2.1.120 | isoprimeverose-producing oligoxyloglucan hydrolase | - |
Aspergillus oryzae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 60 | - |
- |
Aspergillus oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 65 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 289 | - |
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 525 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 869 | - |
lactose | pH 4.5, 60°C | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 4.5 | - |
- |
Aspergillus oryzae |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.2.1.23 | Aspergillus oryzae | expression of the lacA gene is repressed by D-glucose | down |
3.2.1.23 | Aspergillus oryzae | induced in the presence of xyloglucan oligosaccharides | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.23 | physiological function | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA -xyloglucan oligosaccharide complex | Aspergillus oryzae |
3.2.1.120 | physiological function | the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex | Aspergillus oryzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 8.36 | - |
lactose | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 37 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 120 | - |
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
3.2.1.23 | 370 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.5, 60°C | Aspergillus oryzae |