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Literature summary extracted from

  • Moure, V.R.; Sioeberg, C.L.B.; Valdameri, G.; Nji, E.; Oliveira, M.A.S.; Gerdhardt, E.C.M.; Pedrosa, F.O.; Mitchell, D.A.; Seefeldt, L.C.; Huergo, L.F.; Hoegbom, M.; Nordlund, S.; Souza, E.M.
    The ammonium transporter AmtB and the PII signal transduction protein GlnZ are required to inhibit DraG in Azospirillum brasilense (2019), FEBS J., 286, 1214-1229 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.24 expressed in Escherichia coli BL21(DE3) cells Azospirillum brasilense

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.24 hanging drop vapor diffusion method, using 35% (v/v) glycerol ethoxylate and 0.2 M lithium citrate Azospirillum brasilense

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.24 GlnZ the enzyme is partially inhibited by GlnZ. Enzyme inactivation requires the formation of a ternary enzyme-GlnZ-AmtB complex in vitro Azospirillum brasilense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.37 NAD+ + [dinitrogen reductase]-L-arginine Azospirillum brasilense
-
 nicotinamide + [dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine
-
 ?
3.2.2.24 ADP-ribosylated Fe-protein Azospirillum brasilense
-
 ADP-D-ribose + Fe-protein
-
 ?
3.2.2.24 ADP-ribosylated Fe-protein Azospirillum brasilense FP2
-
 ADP-D-ribose + Fe-protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.37 Azospirillum brasilense
-
-
-
3.2.2.24 Azospirillum brasilense A7XNI2
-
-
3.2.2.24 Azospirillum brasilense FP2 A7XNI2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.24 HiTrap heparin column chromatography or His-Trap column chromatography Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.37 NAD+ + [dinitrogen reductase]-L-arginine
-
 Azospirillum brasilense nicotinamide + [dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine
-
 ?
3.2.2.24 ADP-ribosylated Fe-protein
-
 Azospirillum brasilense ADP-D-ribose + Fe-protein
-
 ?
3.2.2.24 ADP-ribosylated Fe-protein
-
 Azospirillum brasilense FP2 ADP-D-ribose + Fe-protein
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.24 ? x * 32000, SDS-PAGE Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
2.4.2.37 dinitrogenase reductase ADP-ribosyl transferase
-
 Azospirillum brasilense
2.4.2.37 DRAT
-
 Azospirillum brasilense
3.2.2.24 dinitrogenase reductase ADP-glycohydrolase
-
 Azospirillum brasilense
3.2.2.24 DRAG
-
 Azospirillum brasilense