EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.12 | expressed in Sf9 insect cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.4.12 | catalytic domain in complex with CMP, sitting drop vapor diffusion method, using 2.08 M disodium malonate pH 5, 0.23 M sodium thiocyanate | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Zn2+ | high millimolar Zn2+ concentration leads to inhibition of enzyme activity | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.12 | 0.262 | - |
CDP-choline | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 0.306 | - |
ADP | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 0.327 | - |
ATP | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 0.348 | - |
ADP-ribose | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 0.391 | - |
CDP-ethanolamine | at pH 5.0 and 37°C | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Zn2+ | contains two zinc ions | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Homo sapiens | Q92484 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.4.12 | glycoprotein | the enzyme has seven sites for glycosylation | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.12 | Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | ADP + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.4.12 | ADP-ribose + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.4.12 | ATP + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.4.12 | CDP-choline + H2O | - |
Homo sapiens | CMP + phosphocholine | - |
? | |
3.1.4.12 | CDP-ethanolamine + H2O | - |
Homo sapiens | CMP + phosphoethanolamine | - |
? | |
3.1.4.12 | additional information | no activity with sphingomyelin or AMP | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.12 | monomer | 1 * 59400, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.12 | SMPDL3A | - |
Homo sapiens |
3.1.4.12 | sphingomyelin phosphodiesterase like 3a | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.12 | 0.374 | - |
ADP | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 0.7357 | - |
ATP | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 1.29 | - |
CDP-choline | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 1.864 | - |
CDP-ethanolamine | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 2.07 | - |
ADP-ribose | at pH 5.0 and 37°C | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.12 | 1.23 | - |
ADP | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 2.26 | - |
ATP | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 4.89 | - |
CDP-choline | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 5.71 | - |
CDP-ethanolamine | at pH 5.0 and 37°C | Homo sapiens | |
3.1.4.12 | 5.96 | - |
ADP-ribose | at pH 5.0 and 37°C | Homo sapiens |