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Literature summary extracted from

  • Fontagne-Faucher, C.; Monsan, P.; Remaud-Simeon, M.; Moulis, C.
    Inventory of the GH70 enzymes encoded by Leuconostoccitreum NRRL B-1299 - identification of three novel alpha-transglucosylases (2015), FEBS J., 282, 2115-2130 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B77 expression in Escherichia coli Leuconostoc citreum
2.4.1.373 expression in Escherichia coli Leuconostoc citreum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B77 Leuconostoc citreum
-
-
-
2.4.1.B77 Leuconostoc citreum NRRL B-1299
-
-
-
2.4.1.373 Leuconostoc citreum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.B77 12
-
pH 9.0, temperature not specified in the publication Leuconostoc citreum
2.4.1.373 12
-
30°C, pH 5.8 Leuconostoc citreum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B77 additional information all the dextrans tested are branched by BRS-A and show 37% alpha-(1->2) linkages Leuconostoc citreum ?
-
?
2.4.1.B77 additional information all the dextrans tested are branched by BRS-A and show 37% alpha-(1->2) linkages Leuconostoc citreum NRRL B-1299 ?
-
?
2.4.1.B77 sucrose + dextran dextran with mean degree of polymerization of 9 Leuconostoc citreum D-fructose + a (1->6)-dextran containing a (1->2)-alpha-D-glucose branch
-
?
2.4.1.B77 sucrose + dextran dextran with mean degree of polymerization of 9 Leuconostoc citreum NRRL B-1299 D-fructose + a (1->6)-dextran containing a (1->2)-alpha-D-glucose branch
-
?
2.4.1.373 sucrose
-
Leuconostoc citreum D-glucose + leucrose + ?
-
?
2.4.1.373 sucrose
-
Leuconostoc citreum NRRL B-1299 D-glucose + leucrose + ?
-
?
2.4.1.373 sucrose + alpha-(1->6)-dextran
-
Leuconostoc citreum D-fructose + alpha-(1->6)-dextran containing a (1->2)-alpha-D-glucose branch
-
?
2.4.1.373 sucrose + alpha-(1->6)-dextran
-
Leuconostoc citreum NRRL B-1299 D-fructose + alpha-(1->6)-dextran containing a (1->2)-alpha-D-glucose branch
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.B77 ? x * 145000, calculated from sequence Leuconostoc citreum
2.4.1.373 ? x * 145000, calculated from sequence Leuconostoc citreum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B77 BRS-A
-
Leuconostoc citreum
2.4.1.373 brsA
-
Leuconostoc citreum