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Literature summary extracted from
- Functional dissection of the three N-terminal general secretory pathway domains and the Walker motifs of the traffic ATPase PilF from Thermus thermophilus (2018), Extremophiles, 22, 461-471 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.4.2.8 | expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.4.2.8 | E718A | catalytic site mutant which is unaffected in ATP binding | Thermus thermophilus |
| 7.4.2.8 | K654A | the Walker A motif site mutant shows about 90% less ATP binding compared to the wild type enzyme | Thermus thermophilus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.4.2.8 | Zn2+ | zinc binding is essential for complex stability and pilus dynamics but not required for pilus biogenesis or natural transformation | Thermus thermophilus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.4.2.8 | 615000 | - |
gel filtration | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.4.2.8 | ATP + H2O + cellular protein[side 1] | Thermus thermophilus | - |
ADP + phosphate + cellular protein[side 2] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.4.2.8 | Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.4.2.8 | Ni-NTA column chromatography and Superdex 200 gel filtration | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.4.2.8 | 0.0227 | - |
wild type enzyme, at pH 8.5, temperature not specified in the publication | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.4.2.8 | ATP + H2O + cellular protein[side 1] | - |
Thermus thermophilus | ADP + phosphate + cellular protein[side 2] | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.4.2.8 | hexamer | 6 * 100000, SDS-PAGE | Thermus thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.4.2.8 | PilF | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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