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Literature summary extracted from

  • Ramirez-Tapia, A.L.; Baylon-Pacheco, L.; Espiritu-Gordillo, P.; Rosales-Encina, J.L.
    Characterization of the protein tyrosine phosphatase PRL from Entamoeba histolytica (2015), Exp. Parasitol., 159, 168-182 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 gene ehprl, RT-PCR cloning, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLyS Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.48 o-vanadate Na3VO4, complete inhibition at 0.6 mM Entamoeba histolytica
3.1.3.48 Sodium fluoride NaF, 30% inhibition at 5 mM, no inhibition at 1-3 mM Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.48 additional information
-
 additional information Michaelis-Menten kinetics Entamoeba histolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.48 cytoplasm
-
 Entamoeba histolytica 5737
-
3.1.3.48 additional information analysis of the cellular localization of EhPRL, overview. EhPRL is localized to the cytoplasm and plasma membrane. When the trophozoites interacts with collagen, EhPRL relocalizes over time to vesicle-like structures. Interaction with fibronectin increases the presence of the enzyme in the cytoplasm Entamoeba histolytica
-
-
3.1.3.48 plasma membrane
-
 Entamoeba histolytica 5886
-
3.1.3.48 vesicle
-
 Entamoeba histolytica 31982
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.48 28000
-
 recombinant His-tagged enzyme, gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.48 [a protein]-tyrosine phosphate + H2O Entamoeba histolytica
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Entamoeba histolytica ATCC 30459
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Entamoeba histolytica HM1:IMSS
-
 [a protein]-tyrosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Entamoeba histolytica A0A0A1EI83
-
-
3.1.3.48 Entamoeba histolytica ATCC 30459 A0A0A1EI83
-
-
3.1.3.48 Entamoeba histolytica HM1:IMSS A0A0A1EI83
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.48 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLyS cell-free extract by nickel affinity chromatography and dialysis Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.48 amoeba
-
 Entamoeba histolytica
-
3.1.3.48 trophozoite EhPRL mRNA expression is upregulated when the trophozoites interacted with collagen but not with fibronectin. Trophozoites recovered from amoebic liver abscesses show higher EhPRL mRNA expression levels than normal trophozoites Entamoeba histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 3-o-methyl fluorescein phosphate + H2O
-
 Entamoeba histolytica 3-o-methyl fluorescein + phosphate
-
 ?
3.1.3.48 3-o-methyl fluorescein phosphate + H2O
-
 Entamoeba histolytica ATCC 30459 3-o-methyl fluorescein + phosphate
-
 ?
3.1.3.48 3-o-methyl fluorescein phosphate + H2O
-
 Entamoeba histolytica HM1:IMSS 3-o-methyl fluorescein + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Entamoeba histolytica [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Entamoeba histolytica ATCC 30459 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Entamoeba histolytica HM1:IMSS [a protein]-tyrosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.48 monomer 1 * 28000, recombinant His-tagged enzyme, SDS-PAGE Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 EhPRL
-
 Entamoeba histolytica
3.1.3.48 PRL
-
 Entamoeba histolytica
3.1.3.48 protein tyrosine phosphatase
-
 Entamoeba histolytica
3.1.3.48 PTP
-
 Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.48 37
-
 assay at Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 6
-
-
 Entamoeba histolytica

Expression

EC Number Organism Comment Expression
3.1.3.48 Entamoeba histolytica enzyme EhPRL mRNA expression is upregulated in trophozoites interacting with type I collagen, but not with fibronectin, or in trophozoites recovered from amoebic liver abscess up

General Information

EC Number General Information Comment Organism
3.1.3.48 evolution the pathogenic enzyme EhPRL protein possesses the classical HCX5R catalytic motif of PTPs and the CAAX box characteristic of the PRL family and exhibits 31-32% homology with the three human PRL isoforms Entamoeba histolytica
3.1.3.48 physiological function EhPRL may play an important role in the biology and adaptive response of the parasite to the host environment during amoebic liver abscess development, thereby participating in the pathogenic mechanism in humans Entamoeba histolytica