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Literature summary extracted from

  • Bendrat, K.; Buckel, W.
    Cloning, sequencing and expression of the gene encoding the carboxytransferase subunit of the biotin-dependent Na+ pump glutaconyl-CoA decarboxylase from Acidaminococcus fermentans in Escherichia coli (1993), Eur. J. Biochem., 211, 697-702 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.4.5 expression in Escherichia coli Acidaminococcus fermentans

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.4.5 N-ethylmaleimide Gcd holoenzyme loses half of its activity within 6 min, whereas the expressed GcdA requires 21 min Acidaminococcus fermentans

Organism

EC Number Organism UniProt Comment Textmining
7.2.4.5 Acidaminococcus fermentans Q06700 and Q9ZAA6 and Q9ZAA7 and Q9ZAA8 Q06700 i.e. subunit GcdA, Q9ZAA6 .e. subunit GcdB, Q9ZAA7 .e. subunit GcdC, Q9ZAA8 .e. subunit GcdD
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7.2.4.5 Acidaminococcus fermentans DSM 20731 Q06700 and Q9ZAA6 and Q9ZAA7 and Q9ZAA8 Q06700 i.e. subunit GcdA, Q9ZAA6 .e. subunit GcdB, Q9ZAA7 .e. subunit GcdC, Q9ZAA8 .e. subunit GcdD
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Subunits

EC Number Subunits Comment Organism
7.2.4.5 ? enzyme is composed of subunits GcdA (65 kDa), GcdB (36 kDa), GcdC (24 kDa) and GcdD (14 kDa), calculated from sequence Acidaminococcus fermentans

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.4.5 Biocytin may substitute for biotin, Km value 13 mM Acidaminococcus fermentans
7.2.4.5 biotin Km value 51 mM Acidaminococcus fermentans

General Information

EC Number General Information Comment Organism
7.2.4.5 physiological function sodium-ion pump glutaconyl-CoA decarboxylase is composed of four subunits: GcdA, the carboxytransferase (65 kDa), GcdB, the carboxylyase (36 kDa), GcdC, the biotin carrier (24 kDa) and GcdD (14 kDa) of unknown function. The GcdA subunit catalyzes the biotin-dependent formation of crotonyl-CoA from glutaconyl-CoA Acidaminococcus fermentans