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Literature summary extracted from

  • Sermsathanaswadi, J.; Baramee, S.; Tachaapaikoon, C.; Pason, P.; Ratanakhanokchai, K.; Kosugi, A.
    The family 22 carbohydrate-binding module of bifunctional xylanase/beta-glucanase Xyn10E from Paenibacillus curdlanolyticus B-6 has an important role in lignocellulose degradation (2017), Enzyme Microb. Technol., 96, 75-84 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.74 expressed in Escherichia coli BL21(DE3) cells Paenibacillus curdlanolyticus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.74 additional information truncation mutant enzymes X-CBM22, X-CBM3 and X-GH10 show 100%, 25.9% and 26.9% activity with barley beta-glucan, respectively, as compared to the wild type enzyme Paenibacillus curdlanolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Paenibacillus curdlanolyticus
-
-
-
3.2.1.74 Paenibacillus curdlanolyticus B-6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 Profinia affinity chromatography protein purification system Paenibacillus curdlanolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 barley beta-glucan + H2O
-
 Paenibacillus curdlanolyticus D-glucose + ?
-
 ?
3.2.1.74 barley beta-glucan + H2O
-
 Paenibacillus curdlanolyticus B-6 D-glucose + ?
-
 ?
3.2.1.74 additional information the recombinant enzyme shows bifunctional degradation activity toward xylan and beta-glucan and also degrades microcrystalline cellulose Paenibacillus curdlanolyticus ?
-
 ?
3.2.1.74 additional information the recombinant enzyme shows bifunctional degradation activity toward xylan and beta-glucan and also degrades microcrystalline cellulose Paenibacillus curdlanolyticus B-6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.74 ? x * 80000, SDS-PAGE Paenibacillus curdlanolyticus
3.2.1.74 ? x * 92804, calculated from amino acid sequence Paenibacillus curdlanolyticus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.74 Xyn10E bifunctional recombinant enzyme with xylanase and beta-glucanase activities Paenibacillus curdlanolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.74 8
-
 Barley beta-glucan at pH 6.0, temperature not specified in the publication Paenibacillus curdlanolyticus