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Literature summary extracted from

  • Rahmati, P.; Sajedi, R.H.; Zamani, P.; Rahmani, H.; Khajeh, K.
    Allosteric properties of Geobacillus maltogenic amylase (2017), Enzyme Microb. Technol., 96, 36-41 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133 expression in Escherichia coli BL21 (DE3)as a His-tagged fusion protein Geobacillus sp. Gh6

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.133 D-glucose in the presence of beta-cyclodextrin the effector has a biphasic effect. While it stimulates the enzyme activity at low concentrations (with a decreasein Hill constant), it acts as allosteric inhibitor at higher concentrations Geobacillus sp. Gh6
3.2.1.133 maltose in the presence of beta-cyclodextrin the effector has a biphasic effect. While it stimulates the enzyme activity at low concentrations (with a decreasein Hill constant), it acts as allosteric inhibitor at higher concentrations Geobacillus sp. Gh6

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Geobacillus sp. Gh6 E0X988
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133
-
 Geobacillus sp. Gh6

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 alpha-cyclodextrin + H2O
-
 Geobacillus sp. Gh6 maltose + ?
-
 ?
3.2.1.133 beta-cyclodextrin + H2O
-
 Geobacillus sp. Gh6 maltose + ?
-
 ?
3.2.1.133 gamma-cyclodextrin + H2O
-
 Geobacillus sp. Gh6 maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.133 More the enzyme shows positive cooperativity between their subunits Geobacillus sp. Gh6

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 MAase
-
 Geobacillus sp. Gh6
3.2.1.133 maltogenic amylase
-
 Geobacillus sp. Gh6

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 60
-
-
 Geobacillus sp. Gh6

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.133 6
-
-
 Geobacillus sp. Gh6

General Information

EC Number General Information Comment Organism
3.2.1.133 metabolism the enzyme has a key role in carbohydrate metabolization Geobacillus sp. Gh6