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Literature summary extracted from
- A new bacterial hydrolase specific for the compatible solutes alpha-D-mannopyranosyl-(1->2)-D-glycerate and alpha-D-glucopyranosyl-(1->2)-D-glycerate (2013), Enzyme Microb. Technol., 52, 77-83 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.208 | 2.62 | - |
2-O-(alpha-D-mannopyranosyl)-D-glycerate | pH 4.5, 70°C | Thermus thermophilus |  |
| 3.2.1.208 | 3.74 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | pH 4.5, 70°C | Thermus thermophilus | |
| 3.2.1.208 | 4.18 | - |
2-O-(alpha-D-glucopyranosyl)-D-glycerate | pH 4.5, 50°C | Rubrobacter radiotolerans | |
| 3.2.1.208 | 6.37 | - |
2-O-(alpha-D-mannopyranosyl)-D-glycerate | pH 4.5, 50°C | Rubrobacter radiotolerans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.208 | 92900 | - |
gel filtration | Rubrobacter radiotolerans |
| 3.2.1.208 | 205800 | - |
gel filtration | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.208 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O | Thermus thermophilus | - |
D-glucopyranose + D-glycerate | - |
? | |
| 3.2.1.208 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O | Rubrobacter radiotolerans | - |
D-glucopyranose + D-glycerate | - |
? | |
| 3.2.1.208 | 2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O | Thermus thermophilus | - |
D-mannopyranose + D-glycerate | - |
? | |
| 3.2.1.208 | 2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O | Rubrobacter radiotolerans | - |
D-mannopyranose + D-glycerate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.208 | Rubrobacter radiotolerans | - |
- |
- |
| 3.2.1.208 | Thermus thermophilus | Q72K04 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.208 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O | - |
Thermus thermophilus | D-glucopyranose + D-glycerate | - |
? | |
| 3.2.1.208 | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O | - |
Rubrobacter radiotolerans | D-glucopyranose + D-glycerate | - |
? | |
| 3.2.1.208 | 2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O | - |
Thermus thermophilus | D-mannopyranose + D-glycerate | - |
? | |
| 3.2.1.208 | 2-O-(alpha-D-mannopyranosyl)-D-glycerate + H2O | - |
Rubrobacter radiotolerans | D-mannopyranose + D-glycerate | - |
? | |
| 3.2.1.208 | additional information | no substrates: mannosylglyceramide, glucosylglycerol, mannosylglucosylglycerate, glucosylglucosylglycerate, 4-nitrophenyl-alpha-D-mannopyranoside and 4-nitrophenyl-alpha-D-glucopyranoside | Thermus thermophilus | ? | - |
? | |
| 3.2.1.208 | additional information | no substrates: mannosylglyceramide, glucosylglycerol, mannosylglucosylglycerate, glucosylglucosylglycerate, 4-nitrophenyl-alpha-D-mannopyranoside and 4-nitrophenyl-alpha-D-glucopyranoside | Rubrobacter radiotolerans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.208 | dimer | 2 * 50400, calculated from sequence | Rubrobacter radiotolerans |
| 3.2.1.208 | tetramer | 4 * 48100, calculated from sequence | Thermus thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.208 | MGH | - |
Thermus thermophilus |
| 3.2.1.208 | MGH | - |
Rubrobacter radiotolerans |
| 3.2.1.208 | RradDRAFT 2972 | - |
Rubrobacter radiotolerans |
| 3.2.1.208 | TT_C0614 | - |
Thermus thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.208 | 50 | - |
- |
Rubrobacter radiotolerans |
| 3.2.1.208 | 70 | - |
- |
Thermus thermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.208 | 30 | - |
no activity below | Thermus thermophilus |
| 3.2.1.208 | 30 | - |
no activity below | Rubrobacter radiotolerans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.208 | 70 | - |
half-life 16.1 h | Thermus thermophilus |
| 3.2.1.208 | 80 | - |
half-life 2.5 h | Thermus thermophilus |
| 3.2.1.208 | 90 | - |
half-life 0.20 h | Thermus thermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.208 | 4.5 | - |
- |
Thermus thermophilus |
| 3.2.1.208 | 4.5 | - |
- |
Rubrobacter radiotolerans |
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Release 2023.1 (January 2023)
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