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Literature summary extracted from

  • Chen, A.; Xu, T.; Ge, Y.; Wang, L.; Tang, W.; Li, S.
    Hydrogen-bond-based protein engineering for the acidic adaptation of Bacillus acidopullulyticus pullulanase (2019), Enzyme Microb. Technol., 124, 79-83 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.41 food industry break down of starch for the production of high-glucose syrup. The L627R mutant enzyme may be suitable for industrial application because its shortened reaction time translates to reduced energy consumption Bacillus acidopullulyticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli BL21(DE3) Bacillus acidopullulyticus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.41 L627R the pH optimum of the mutant enzyme shifts from 5.0 to 4.0, and its relative activity at pH 4.0 is 117% that of the wide-type enzyme. The L627R mutant exhibits increased tolerance against acid-mediated denaturation, and its maximum D-glucose content (97.4%) is obtained after 40 h incubation, which is shorter by 10 h compared with the time required by the wild-type enzyme to produce a comparable amount of the monosaccharide. The L627R mutant may be suitable for industrial application because its shortened reaction time translates to reduced energy consumption Bacillus acidopullulyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 additional information
-
 pullulan Km-value: 0.61 mg/ml, pH 5.0, 60°C, mutant enzyme L627R Bacillus acidopullulyticus
3.2.1.41 additional information
-
 pullulan Km-value: 0.93 mg/ml, pH 5.0, 60°C, wild-type enzyme Bacillus acidopullulyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Bacillus acidopullulyticus Ax203843.1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Bacillus acidopullulyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.41 984.2
-
 substrate: pullulan, pH 5.0, 60°C, wild-type enzyme Bacillus acidopullulyticus
3.2.1.41 1178.5
-
 substrate: pullulan, pH 5.0, 60°C, mutant enzyme L627R Bacillus acidopullulyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 pullulan + H2O
-
 Bacillus acidopullulyticus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 60
-
 assay at Bacillus acidopullulyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.41 770.4
-
 pullulan pH 5.0, 60°C, mutant enzyme L627R Bacillus acidopullulyticus
3.2.1.41 977.3
-
 pullulan pH 5.0, 60°C, wild-type enzyme Bacillus acidopullulyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 5
-
-
 Bacillus acidopullulyticus