Any feedback?
Literature summary extracted from
- Improvement of enzymatic properties of Rhizopus oryzae alpha-amylase by site-saturation mutagenesis of histidine 286 (2018), Enzyme Microb. Technol., 117, 96-102 .
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | H286A | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286C | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286D | site-directed mutagenesis, the mutant shows a decreased optimum pH compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286E | site-directed mutagenesis, the mutant shows a 6.43fold increase in half-life at pH 4.5 and an decreased pH optimum compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286F | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286G | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286I | site-directed mutagenesis, mutant H286I shows a 1.5fold increase in half-life at 55°C compared to wild-type, and the mutant shows an increased optimum temperature and a decreased pH optimum compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286K | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286L | site-directed mutagenesis, the mutant shows an increased optimum temperature and a decreased pH optimum compared to wild-type, the ability to form maltose from soluble starch is significantly improved, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286M | site-directed mutagenesis, the mutant shows an inproved ability to form maltose from soluble starch compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286N | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286P | site-directed mutagenesis, the mutant shows an increased optimum temperature compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286Q | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286R | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286S | site-directed mutagenesis, the mutant shows an increased optimum temperature compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286T | site-directed mutagenesis, the mutant shows an increased optimum temperature compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286V | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286W | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | H286Y | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| 3.2.1.1 | additional information | activities and half-lives of mutant enzymes compared to wild-type enzyme at different pH values, and molecular docking of maltotriose to wild-type and mutant enzymes, overview | Rhizopus arrhizus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview | Rhizopus arrhizus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | starch + H2O | Rhizopus arrhizus | - |
D-glucose + maltose + maltotriose | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Rhizopus arrhizus | A0A1P8L1E1 | i.e. Rhizopus arrhizus var. delemar | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | starch + H2O | - |
Rhizopus arrhizus | D-glucose + maltose + maltotriose | - |
? | |
| 3.2.1.1 | starch + H2O | quantitative analysis of reaction products of wild-type and H286 mutant enzymes, overview | Rhizopus arrhizus | D-glucose + maltose + maltotriose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ROAmy | - |
Rhizopus arrhizus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 50 | - |
wild-type enzyme | Rhizopus arrhizus |
| 3.2.1.1 | 55 | - |
mutants H286 L, H286I, H286S and H286 T | Rhizopus arrhizus |
| 3.2.1.1 | 60 | - |
mutant H286P | Rhizopus arrhizus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 4.5 | - |
mutant H286E | Rhizopus arrhizus |
| 3.2.1.1 | 5 | - |
mutants H286L, H286I, and H286D | Rhizopus arrhizus |
| 3.2.1.1 | 5.5 | - |
wild-type enzyme | Rhizopus arrhizus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | additional information | molecular docking of maltotriose to wild-type and mutant enzymes, overview | Rhizopus arrhizus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
