EC Number | Cloned (Comment) | Organism |
---|---|---|
7.6.2.8 | recombinant expression of ECF-CbrT complements in an Escherichia coli strain lacking its endogenous vitamin B12 transporter (Escherichia coli strain DELTAFEC, with btuF and btuC also deleted , thus abolished endogenous Cbl-uptake, from parental strain JW0154). Recombinant ECF-CbrT supports cobalamin-dependent growth of an Escherichia coli deletion strain | Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
7.6.2.8 | purified ECF-CbrT complex in detergent (n-dodecyl-beta-D-maltopyranoside) solution, sitting drop vapour diffusion method, mixing of protein solution containing 50 mM HEPES pH 8, 150 mM NaCl, 1% polyoxyethylene(10)dodecyl ether, with precipitant solution containing 0.2 M KCl, 0.1 M sodium citrate, pH 5.5, 37% v/v pentaerythritol propoxylate, X-ray diffraction structure determination and analysis at 3.4 A resolution, molecular replacement with the structure of the folate transporter, ECF-FolT2, from Lactobacillus delbrueckii as a search model | Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.6.2.8 | adenosyl-cobalamin | is less effective causing about 25% inhibition of cobalmin uptake | Lactobacillus delbrueckii subsp. bulgaricus | |
7.6.2.8 | cobinamide | addition of a 250fold excess of cobalamin decreases the uptake of radiolabeled cobalamin to about 25% | Lactobacillus delbrueckii subsp. bulgaricus | |
7.6.2.8 | cyano-cobalamin | a 250fold excess of unlabeled CN-Cbl inhibits the uptake of the radiolabeled substrate almost completely | Lactobacillus delbrueckii subsp. bulgaricus | |
7.6.2.8 | hydroxycobalamin | inhibits uptake to a similar extent as cyano-cobalamin | Lactobacillus delbrueckii subsp. bulgaricus | |
7.6.2.8 | methylcobalamin | inhibits uptake to a similar extent as cyano-cobalamin | Lactobacillus delbrueckii subsp. bulgaricus | |
7.6.2.8 | additional information | hemin does not compete with cobalamin-uptake | Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.6.2.8 | additional information | - |
additional information | binding kinetics of substrates and competing ligands | Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.6.2.8 | membrane | integral membrane enzyme | Lactobacillus delbrueckii subsp. bulgaricus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.6.2.8 | Mg2+ | required | Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus | - |
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | - |
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953 | - |
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus JCM 1002 | - |
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778 | - |
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 | - |
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus | - |
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | - |
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953 | - |
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus JCM 1002 | - |
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778 | - |
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 | - |
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.6.2.8 | Lactobacillus delbrueckii subsp. bulgaricus | Q1GBI8 | - |
- |
7.6.2.8 | Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 | Q1GBI8 | - |
- |
7.6.2.8 | Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | Q1GBI8 | - |
- |
7.6.2.8 | Lactobacillus delbrueckii subsp. bulgaricus JCM 1002 | Q1GBI8 | - |
- |
7.6.2.8 | Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953 | Q1GBI8 | - |
- |
7.6.2.8 | Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778 | Q1GBI8 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus | ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953 | ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002 | ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778 | ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + cobinamide-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 | ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus | ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953 | ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002 | ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778 | ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | - |
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 | ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
7.6.2.8 | additional information | hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter | Lactobacillus delbrueckii subsp. bulgaricus | ? | - |
? | |
7.6.2.8 | additional information | hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter | Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | ? | - |
? | |
7.6.2.8 | additional information | hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter | Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953 | ? | - |
? | |
7.6.2.8 | additional information | hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter | Lactobacillus delbrueckii subsp. bulgaricus JCM 1002 | ? | - |
? | |
7.6.2.8 | additional information | hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter | Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778 | ? | - |
? | |
7.6.2.8 | additional information | hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter | Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.6.2.8 | More | ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family | Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.6.2.8 | Cbl transporter | - |
Lactobacillus delbrueckii subsp. bulgaricus |
7.6.2.8 | cobalamin-specific ECF-type ABC transporter | - |
Lactobacillus delbrueckii subsp. bulgaricus |
7.6.2.8 | ECF-CbrT | - |
Lactobacillus delbrueckii subsp. bulgaricus |
7.6.2.8 | ECF-type ABC transporter for vitamin B12 | - |
Lactobacillus delbrueckii subsp. bulgaricus |
7.6.2.8 | energy coupling factor (ECF-) type ABC transporter | - |
Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.6.2.8 | 30 | - |
assay at | Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.6.2.8 | 7.5 | - |
assay at | Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.6.2.8 | ATP | - |
Lactobacillus delbrueckii subsp. bulgaricus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.6.2.8 | evolution | ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family | Lactobacillus delbrueckii subsp. bulgaricus |
7.6.2.8 | physiological function | cobalamin-specific ECF-type ABC transporter from Lactobacillus delbrueckii, ECF-CbrT, mediates the specific, ATP-dependent uptake of cobalamin. Cobalamin (vitamin B12) is the most complex B-type vitamin and is synthetized exclusively in a limited number of prokaryotes. Its biologically active variants contain rare organometallic bonds, which are used by enzymes in a variety of central metabolic pathways such as L-methionine synthesis and ribonucleotide reduction. Enzyme ECF-CbrT catalyzes ATP-dependent transport of cobalamin and cobinamide | Lactobacillus delbrueckii subsp. bulgaricus |