BRENDA - Enzyme Database

Functional and structural characterization of an ECF-type ABC transporter for vitamin B12

Santos, J.A.; Rempel, S.; Mous, S.T.; Pereira, C.T.; Ter Beek, J.; de Gier, J.W.; Guskov, A.; Slotboom, D.J.; eLife 7, e35828 (2018)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
7.6.2.8
recombinant expression of ECF-CbrT complements in an Escherichia coli strain lacking its endogenous vitamin B12 transporter (Escherichia coli strain DELTAFEC, with btuF and btuC also deleted , thus abolished endogenous Cbl-uptake, from parental strain JW0154). Recombinant ECF-CbrT supports cobalamin-dependent growth of an Escherichia coli deletion strain
Lactobacillus delbrueckii subsp. bulgaricus
Crystallization (Commentary)
EC Number
Crystallization
Organism
7.6.2.8
purified ECF-CbrT complex in detergent (n-dodecyl-beta-D-maltopyranoside) solution, sitting drop vapour diffusion method, mixing of protein solution containing 50 mM HEPES pH 8, 150 mM NaCl, 1% polyoxyethylene(10)dodecyl ether, with precipitant solution containing 0.2 M KCl, 0.1 M sodium citrate, pH 5.5, 37% v/v pentaerythritol propoxylate, X-ray diffraction structure determination and analysis at 3.4 A resolution, molecular replacement with the structure of the folate transporter, ECF-FolT2, from Lactobacillus delbrueckii as a search model
Lactobacillus delbrueckii subsp. bulgaricus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.8
cobinamide
addition of a 250fold excess of cobalamin decreases the uptake of radiolabeled cobalamin to about 25%
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
cyano-cobalamin
a 250fold excess of unlabeled CN-Cbl inhibits the uptake of the radiolabeled substrate almost completely
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
hydroxycobalamin
inhibits uptake to a similar extent as cyano-cobalamin
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
methylcobalamin
inhibits uptake to a similar extent as cyano-cobalamin
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
S-adenosyl-cobalamin
is less effective causing about 25% inhibition of cobalmin uptake
Lactobacillus delbrueckii subsp. bulgaricus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.6.2.8
additional information
-
additional information
binding kinetics of substrates and competing ligands
Lactobacillus delbrueckii subsp. bulgaricus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.8
membrane
integral membrane enzyme
Lactobacillus delbrueckii subsp. bulgaricus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.8
Mg2+
required
Lactobacillus delbrueckii subsp. bulgaricus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.6.2.8
Lactobacillus delbrueckii subsp. bulgaricus
Q1GBI8
-
-
7.6.2.8
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
Q1GBI8
-
-
7.6.2.8
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
Q1GBI8
-
-
7.6.2.8
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
Q1GBI8
-
-
7.6.2.8
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
Q1GBI8
-
-
7.6.2.8
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
Q1GBI8
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
7.6.2.8
More
ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family
Lactobacillus delbrueckii subsp. bulgaricus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7.6.2.8
30
-
assay at
Lactobacillus delbrueckii subsp. bulgaricus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6.2.8
7.5
-
assay at
Lactobacillus delbrueckii subsp. bulgaricus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.6.2.8
ATP
-
Lactobacillus delbrueckii subsp. bulgaricus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.6.2.8
recombinant expression of ECF-CbrT complements in an Escherichia coli strain lacking its endogenous vitamin B12 transporter (Escherichia coli strain DELTAFEC, with btuF and btuC also deleted , thus abolished endogenous Cbl-uptake, from parental strain JW0154). Recombinant ECF-CbrT supports cobalamin-dependent growth of an Escherichia coli deletion strain
Lactobacillus delbrueckii subsp. bulgaricus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.6.2.8
ATP
-
Lactobacillus delbrueckii subsp. bulgaricus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
7.6.2.8
purified ECF-CbrT complex in detergent (n-dodecyl-beta-D-maltopyranoside) solution, sitting drop vapour diffusion method, mixing of protein solution containing 50 mM HEPES pH 8, 150 mM NaCl, 1% polyoxyethylene(10)dodecyl ether, with precipitant solution containing 0.2 M KCl, 0.1 M sodium citrate, pH 5.5, 37% v/v pentaerythritol propoxylate, X-ray diffraction structure determination and analysis at 3.4 A resolution, molecular replacement with the structure of the folate transporter, ECF-FolT2, from Lactobacillus delbrueckii as a search model
Lactobacillus delbrueckii subsp. bulgaricus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.8
cobinamide
addition of a 250fold excess of cobalamin decreases the uptake of radiolabeled cobalamin to about 25%
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
cyano-cobalamin
a 250fold excess of unlabeled CN-Cbl inhibits the uptake of the radiolabeled substrate almost completely
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
hydroxycobalamin
inhibits uptake to a similar extent as cyano-cobalamin
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
methylcobalamin
inhibits uptake to a similar extent as cyano-cobalamin
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
S-adenosyl-cobalamin
is less effective causing about 25% inhibition of cobalmin uptake
Lactobacillus delbrueckii subsp. bulgaricus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.6.2.8
additional information
-
additional information
binding kinetics of substrates and competing ligands
Lactobacillus delbrueckii subsp. bulgaricus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.8
membrane
integral membrane enzyme
Lactobacillus delbrueckii subsp. bulgaricus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.8
Mg2+
required
Lactobacillus delbrueckii subsp. bulgaricus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus JCM 1002
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
?
-
-
-
-
7.6.2.8
additional information
hemin does not compete with cobalamin-uptake. Although promiscuous among cobalamin variants and cobalamin precursors, ECF-CbrT is a dedicated vitamin B12 transporter
750412
Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.6.2.8
More
ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family
Lactobacillus delbrueckii subsp. bulgaricus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7.6.2.8
30
-
assay at
Lactobacillus delbrueckii subsp. bulgaricus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6.2.8
7.5
-
assay at
Lactobacillus delbrueckii subsp. bulgaricus
General Information
EC Number
General Information
Commentary
Organism
7.6.2.8
evolution
ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
physiological function
cobalamin-specific ECF-type ABC transporter from Lactobacillus delbrueckii, ECF-CbrT, mediates the specific, ATP-dependent uptake of cobalamin. Cobalamin (vitamin B12) is the most complex B-type vitamin and is synthetized exclusively in a limited number of prokaryotes. Its biologically active variants contain rare organometallic bonds, which are used by enzymes in a variety of central metabolic pathways such as L-methionine synthesis and ribonucleotide reduction. Enzyme ECF-CbrT catalyzes ATP-dependent transport of cobalamin and cobinamide
Lactobacillus delbrueckii subsp. bulgaricus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
7.6.2.8
evolution
ECF-transporters are multi-subunit membrane complexes that consist of two ATPases, similar to the ATPases of ABC transporters, and two membrane embedded proteins, not related to any other protein family
Lactobacillus delbrueckii subsp. bulgaricus
7.6.2.8
physiological function
cobalamin-specific ECF-type ABC transporter from Lactobacillus delbrueckii, ECF-CbrT, mediates the specific, ATP-dependent uptake of cobalamin. Cobalamin (vitamin B12) is the most complex B-type vitamin and is synthetized exclusively in a limited number of prokaryotes. Its biologically active variants contain rare organometallic bonds, which are used by enzymes in a variety of central metabolic pathways such as L-methionine synthesis and ribonucleotide reduction. Enzyme ECF-CbrT catalyzes ATP-dependent transport of cobalamin and cobinamide
Lactobacillus delbrueckii subsp. bulgaricus