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Literature summary extracted from

  • Subramani, S.; Perdreau-Dahl, H.; Morth, J.P.
    The magnesium transporter A is activated by cardiolipin and is highly sensitive to free magnesium in vitro (2016), eLife, 5, e11407 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.2.14 cardiolipin
-
 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.14 expressed in Escherichia coli C43(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.14 D373N inactive Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.14 ADP-AlF4- less than 10% residual activity at 1 mM Escherichia coli
7.2.2.14 AlF4- less than 10% residual activity at 1 mM Escherichia coli
7.2.2.14 Ca2+
-
 Escherichia coli
7.2.2.14 Co2+
-
 Escherichia coli
7.2.2.14 Mg2+ the enzyme is highly sensitive to free magnesium in vitro and is strongly inhibited above 1 mM Escherichia coli
7.2.2.14 MgF42- about 70% residual activity at 1 mM Escherichia coli
7.2.2.14 Mn2+
-
 Escherichia coli
7.2.2.14 additional information not inhibited by azide Escherichia coli
7.2.2.14 Ni2+ non-competitive inhibitor Escherichia coli
7.2.2.14 vanadate about 25% residual activity at 1 mM Escherichia coli
7.2.2.14 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.2.14 0.01
-
 Mg2+[side 1] with 3 or 6 mM ATP, at pH 7.0 and 37°C Escherichia coli
7.2.2.14 0.015
-
 Mg2+[side 1] with 1 mM ATP, at pH 7.0 and 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.14 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.14 Mg2+ the enzyme is activated when the free Mg2+ concentration is reduced below 0.01 mM Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.14 ATP + H2O + Mg2+[side 1] Escherichia coli
-
 ADP + phosphate + Mg2+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.14 Escherichia coli P0ABB8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
7.2.2.14 phosphoprotein
-
 Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.14 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.14 ATP + H2O + Mg2+[side 1]
-
 Escherichia coli ADP + phosphate + Mg2+[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.2.14 monomer 1 * 100000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
7.2.2.14 magnesium transporter A
-
 Escherichia coli
7.2.2.14 MgtA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.2.2.14 22
-
 Mg2+[side 1] with 3 or 6 mM ATP, at pH 7.0 and 37°C Escherichia coli
7.2.2.14 23
-
 Mg2+[side 1] with 1 mM ATP, at pH 7.0 and 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.2.14 7.2
-
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.2.2.14 0.005
-
 at pH 7.0 and 37°C Escherichia coli Zn2+
7.2.2.14 0.037
-
 at pH 7.0 and 37°C Escherichia coli Co2+
7.2.2.14 0.1
-
 at pH 7.0 and 37°C Escherichia coli Ni2+
7.2.2.14 0.5
-
 at pH 7.0 and 37°C Escherichia coli Ca2+
7.2.2.14 1
-
 at pH 7.0 and 37°C Escherichia coli Mn2+

General Information

EC Number General Information Comment Organism
7.2.2.14 metabolism the enzyme acts as a sensor as well as a transporter of Mg2+ Escherichia coli