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Literature summary extracted from

  • Romero-Borbon, E.; Grajales-Hernandez, D.; Armendariz-Ruiz, M.; Ramirez-Velasco, L.; Rodriguez-Gonzalez, J.; Cira-Chavez, L.; Estrada-Alvarado, M.; Mateos-Diaz, J.
    Type C feruloyl esterase from Aspergillus ochraceus A butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system (2018), Electron. J. Biotechnol., 35, 1-9 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 2-mercaptoethanol 1 mM, 83% of initial activity Aspergillus ochraceus
3.1.1.73 Cu2+ 1 mM, 55% of initial activity Aspergillus ochraceus
3.1.1.73 Hg2+ 1 mM, 10% of initial activity Aspergillus ochraceus
3.1.1.73 PMSF 1 mM, no residual activity Aspergillus ochraceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.53
-
 methyl caffeate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 0.58
-
 methyl p-coumarate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 0.64
-
 methyl ferulate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 1.11
-
 methyl sinapinate pH 7.2, 30°C Aspergillus ochraceus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 Ca2+ 1 mM, 209% of initial activity Aspergillus ochraceus
3.1.1.73 Mg2+ 1 mM, 177% of initial activity Aspergillus ochraceus
3.1.1.73 Mn2+ 1 mM, 143% of initial activity Aspergillus ochraceus
3.1.1.73 Zn2+ 1 mM, 112% of initial activity Aspergillus ochraceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 26500
-
 gel filtration Aspergillus ochraceus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.73 Butanol maximum initial synthetic rate of 2.8 nmol/min for transesterification reaction to yiled butyl ferulate is reached at 25% (v/v) butanol, using an isooctane:butanol:water relation of 73:25:2 Aspergillus ochraceus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus ochraceus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Aspergillus ochraceus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 57.9
-
 substrate methyl ferulate, pH 7.2, 30°C Aspergillus ochraceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus ochraceus methanol + caffeate
-
 ?
3.1.1.73 methyl ferulate + butanol
-
 Aspergillus ochraceus methanol + butyl ferulate
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus ochraceus methanol + ferulate
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Aspergillus ochraceus methanol + p-coumarate
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Aspergillus ochraceus methanol + sinapinate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 34600, SDS-PAGE Aspergillus ochraceus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FaeC
-
 Aspergillus ochraceus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
-
 Aspergillus ochraceus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 2306
-
 methyl caffeate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 2306
-
 methyl sinapinate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 2710
-
 methyl p-coumarate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 3344
-
 methyl ferulate pH 7.2, 30°C Aspergillus ochraceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6.5
-
-
 Aspergillus ochraceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 10
-
 40% of maximum activity Aspergillus ochraceus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 2078
-
 methyl sinapinate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 4352
-
 methyl caffeate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 4673
-
 methyl p-coumarate pH 7.2, 30°C Aspergillus ochraceus
3.1.1.73 5226
-
 methyl ferulate pH 7.2, 30°C Aspergillus ochraceus