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Literature summary extracted from

  • Nadaroglu, H.; Sonmez, Z.
    Purification of an endo-beta 1,4-mannanase from Clitocybe geotropa and immobilization on chitosan-coated magnetite nanoparticles Application for fruit juices (2016), Dig. J. Nanomater. Biostruct., 11, 685-697 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.78 food industry the enzyme is used for clarification of fruit juices such as grape, peach, orange and pomegranate juices Infundibulicybe geotropa

General Stability

EC Number General Stability Organism
3.2.1.78 the activity of enzyme immobilized onto chitosan coated magnetic nanoparticles is lost 21.4% after 10 cycles of reuse Infundibulicybe geotropa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Mg2+ 89.7% residual activity at 5 mM Infundibulicybe geotropa
3.2.1.78 Zn2+ 90.9% residual activity at 5 mM Infundibulicybe geotropa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 Ca2+ 133.4% activity at 5 mM Infundibulicybe geotropa
3.2.1.78 Cu2+ 187.9% activity at 1 mM Infundibulicybe geotropa
3.2.1.78 Fe2+ 118.2% activity at 5 mM Infundibulicybe geotropa
3.2.1.78 Hg2+ 454.6% activity at 5 mM Infundibulicybe geotropa
3.2.1.78 Mg2+ 109% activity at 1 mM Infundibulicybe geotropa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Infundibulicybe geotropa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
Infundibulicybe geotropa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 ammonium sulfate precipitation, DEAE-Sephadex column chromatography, and Sephacryl S-200 gel filtration Infundibulicybe geotropa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 0.475
-
crude extract, at pH 5.0 and 50°C Infundibulicybe geotropa
3.2.1.78 83.81
-
after 178.4fold purification, at pH 5.0 and 50°C Infundibulicybe geotropa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 casein + H2O
-
Infundibulicybe geotropa ?
-
?
3.2.1.78 cellulose + H2O
-
Infundibulicybe geotropa ?
-
?
3.2.1.78 locust bean gum + H2O highest activity Infundibulicybe geotropa mannose + ?
-
?
3.2.1.78 pectin + H2O
-
Infundibulicybe geotropa ?
-
?
3.2.1.78 sodium phytate + H2O
-
Infundibulicybe geotropa ?
-
?
3.2.1.78 starch + H2O
-
Infundibulicybe geotropa ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 39500, SDS-PAGE Infundibulicybe geotropa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-beta 1,4-mannanase
-
Infundibulicybe geotropa
3.2.1.78 mannan endo-1.4-beta-D-mannosidase
-
Infundibulicybe geotropa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 50
-
free enzyme Infundibulicybe geotropa
3.2.1.78 60
-
immobilized enzyme Infundibulicybe geotropa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 5
-
-
Infundibulicybe geotropa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 3.5 8 the immobilized enzyme exhibits more than 50% activity between pH 3.5 and 8.0 Infundibulicybe geotropa
3.2.1.78 4 6.5 the free enzyme exhibits more than 50% activity between pH 4.0 and 6.5 Infundibulicybe geotropa