EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.41 | D332H/D398Y | mutant enzyme shows remarkable improvement of thermal stability in higher temperature range (above 55 °C). The best temperature of the relative activity moves to 60°C. The activity performance in the middle temperature range (40 to 55°C) is worse than that of the wild type pullulanase | Bacillus deramificans |
3.2.1.41 | D332H/D398Y/V390N | the activity performance of mutations D332H/D398Y/V390N is better than that of mutations D332H/D398Y/V390S in all temperature range from 40°C to 65°C | Bacillus deramificans |
3.2.1.41 | D332H/D398Y/V390S | the activity performance of the mutant enzyme is better than the wild pullulanase-BDPulA in all temperature range from 40°C to 65°C. In the temperature range lower than 55°C the activity is worse than mutation V390S alone, and in the temperature range higher than 55°C the activity of the mutant enzyme is worse than the mutation D332H/D398Y, but better than the wild type enzyme and V390S-mutated BDPulA | Bacillus deramificans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.41 | Bacillus deramificans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.41 | - |
Bacillus deramificans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.41 | pullulan + H2O | - |
Bacillus deramificans | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.41 | BDPulA324 | - |
Bacillus deramificans |
3.2.1.41 | CAC60157 | locus name | Bacillus deramificans |