Literature summary extracted from
Steward, M.; Berezovskaya, Y.; Zhou, H.; Shediac, R.; Sun, C.; Miller, N.; Rendle, P.M.
Recombinant, truncated B. circulans keratanase-II Description and characterisation of a novel enzyme for use in measuring urinary keratan sulphate levels via LC-MS/MS in Morquio A syndrome (2015), Clin. Biochem., 48, 796-802 .
Application
EC Number |
Application |
Comment |
Organism |
---|
3.2.1.B43 |
medicine |
an LC-MS/MS assay utilising keratan sulphate disaccharides derived from keratanase-II digestion provides a sensitive and specific means for quantitation of urinary keratan sulfate, a screening biomarker for Morquio A. The C-terminally truncated, recombinant keratanase-II meets all performance requirements and can be produced in a rapid and reproducible manner |
Niallia circulans |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.B43 |
expression of a C-terminally truncated, recombinant keratanase-II in Escherichia coli. The truncated enzyme is designed to remove the domains predicted to be unnecessary for catalytic activity and detrimental to recombinant expression in Escherichia coli |
Niallia circulans |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.B43 |
Niallia circulans |
Q7X0Z0 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.B43 |
C-terminally truncated, recombinant keratanase-II is purified to more than 98% homogeneity |
Niallia circulans |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.B43 |
keratanase II |
- |
Niallia circulans |
3.2.1.B43 |
KSase II |
- |
Niallia circulans |