Literature summary extracted from

Steward, M.; Berezovskaya, Y.; Zhou, H.; Shediac, R.; Sun, C.; Miller, N.; Rendle, P.M.
Recombinant, truncated B. circulans keratanase-II Description and characterisation of a novel enzyme for use in measuring urinary keratan sulphate levels via LC-MS/MS in Morquio A syndrome (2015), Clin. Biochem., 48, 796-802 .

Application

EC Number	Application	Comment	Organism
3.2.1.B43	medicine	an LC-MS/MS assay utilising keratan sulphate disaccharides derived from keratanase-II digestion provides a sensitive and specific means for quantitation of urinary keratan sulfate, a screening biomarker for Morquio A. The C-terminally truncated, recombinant keratanase-II meets all performance requirements and can be produced in a rapid and reproducible manner	Niallia circulans

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.B43	expression of a C-terminally truncated, recombinant keratanase-II in Escherichia coli. The truncated enzyme is designed to remove the domains predicted to be unnecessary for catalytic activity and detrimental to recombinant expression in Escherichia coli	Niallia circulans

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.B43	Niallia circulans	Q7X0Z0	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.B43	C-terminally truncated, recombinant keratanase-II is purified to more than 98% homogeneity	Niallia circulans

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.B43	keratanase II	-	Niallia circulans
3.2.1.B43	KSase II	-	Niallia circulans

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Release 2023.1 (January 2023)