Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jamek, S.B.; Muschiol, J.; Holck, J.; Zeuner, B.; Busk, P.K.; Mikkelsen, J.D.; Meyer, A.S.
    Loop protein engineering for improved transglycosylation activity of a beta-N-acetylhexosaminidase (2018), ChemBioChem, 19, 1858-1865 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in Escherichia coli T7 Express lysY cells uncultured Bacteroidetes bacterium
3.2.1.52 expressed in Escherichia coli T7 Express lysY cells uncultured Actinomycetales bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.043
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Actinomycetales bacterium
3.2.1.52 0.119
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Bacteroidetes bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 uncultured Actinomycetales bacterium A0A0E3XI19
-
-
3.2.1.52 uncultured Bacteroidetes bacterium A0A0E3XHF8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 His-Trap column chromatography uncultured Bacteroidetes bacterium
3.2.1.52 His-Trap column chromatography uncultured Actinomycetales bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 uncultured Bacteroidetes bacterium 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 uncultured Actinomycetales bacterium 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + beta-lactose transglycosylation uncultured Bacteroidetes bacterium lacto-N-triose II + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + beta-lactose transglycosylation uncultured Actinomycetales bacterium lacto-N-triose II + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 53000, SDS-PAGE uncultured Actinomycetales bacterium
3.2.1.52 ? x * 60000, SDS-PAGE uncultured Bacteroidetes bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 Hex1
-
 uncultured Actinomycetales bacterium
3.2.1.52 Hex2
-
 uncultured Bacteroidetes bacterium
3.2.1.52 hexosaminidase
-
 uncultured Bacteroidetes bacterium
3.2.1.52 hexosaminidase
-
 uncultured Actinomycetales bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 11.6
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Bacteroidetes bacterium
3.2.1.52 19.4
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Actinomycetales bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.52 96.6
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Bacteroidetes bacterium
3.2.1.52 460
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide at pH 8.0 and 25°C uncultured Actinomycetales bacterium