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Literature summary extracted from
- Invitro evaluation of the catalytic activity of paraoxonases and phosphotriesterases predicts the enzyme circulatory levels required for invivo protection against organophosphate intoxications (2016), Chem. Biol. Interact., 259, 252-256 .
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | additional information | generation of wild-type-like-G3C9-derived PON1 variants 2D8, IIG1, VIID2 and PG11 | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | additional information | - |
additional information | classical Michaelis-Menten kinetics | Pseudomonas sp. | |
| 3.1.8.1 | additional information | - |
additional information | classical Michaelis-Menten kinetics | Agrobacterium tumefaciens | |
| 3.1.8.1 | additional information | - |
additional information | classical Michaelis-Menten kinetics | Homo sapiens | |
| 3.1.8.1 | additional information | - |
additional information | classical Michaelis-Menten kinetics | Brevundimonas diminuta |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.8.1 | dichlorvos + H2O | Agrobacterium tumefaciens | - |
2,2-dichloroethenol + dimethyl hydrogen phosphate | - |
? |  |
| 3.1.8.1 | methyl-parathion + H2O | Agrobacterium tumefaciens | - |
dimethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | O,O-diethyl fluorophosphate + H2O | Pseudomonas sp. | activity of EC 3.1.8.2 | diethylphosphate + fluoride | - |
? | |
| 3.1.8.1 | paraoxon + H2O | Pseudomonas sp. | - |
diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | paraoxon + H2O | Agrobacterium tumefaciens | - |
diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | paraoxon + H2O | Homo sapiens | - |
diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | paraoxon + H2O | Brevundimonas diminuta | - |
diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | parathion + H2O | Pseudomonas sp. | - |
diethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | parathion + H2O | Agrobacterium tumefaciens | - |
diethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | parathion + H2O | Homo sapiens | - |
diethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | parathion + H2O | Brevundimonas diminuta | - |
diethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | SP-CMP + H2O | Homo sapiens | SP-CMP is the toxic SP enantiomer of a cyclosarin surrogate in which the fluoride leaving group is replaced by a coumarin derivative | ? | - |
? | |
| 3.1.8.1 | tabun + H2O | Pseudomonas sp. | - |
cyanophosphonic acid dimethylamide + ethanol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.8.1 | Agrobacterium tumefaciens | Q93LD7 | i.e. Rhizobium radiobacter or Agrobacterium tumefaciens | - |
| 3.1.8.1 | Brevundimonas diminuta | P0A434 | - |
- |
| 3.1.8.1 | Homo sapiens | P27169 | - |
- |
| 3.1.8.1 | Pseudomonas sp. | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 0.083 | - |
substrate O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, pH 8.0, 25°C, enzyme mutant C23 administered in guinea pigs in vivo | Brevundimonas diminuta |
| 3.1.8.1 | 0.567 | - |
substrate cyclosarin, pH 8.0, 25°C, enzyme administered in guinea pigs in vivo | Brevundimonas diminuta |
| 3.1.8.1 | 1.4 | - |
substrate cyclosarin, pH 8.0, 37°C, enzyme administered in guinea pigs in vivo | Brevundimonas diminuta |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.8.1 | cyclohexylmethylphosphonofluoridate + H2O | i.e. cyclosarin, activity of 3.1.8.2 | Brevundimonas diminuta | ? | - |
? | |
| 3.1.8.1 | dichlorvos + H2O | - |
Agrobacterium tumefaciens | 2,2-dichloroethenol + dimethyl hydrogen phosphate | - |
? | |
| 3.1.8.1 | methyl-parathion + H2O | - |
Agrobacterium tumefaciens | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | O,O-diethyl fluorophosphate + H2O | activity of EC 3.1.8.2 | Pseudomonas sp. | diethylphosphate + fluoride | - |
? | |
| 3.1.8.1 | O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O | i.e. VX | Brevundimonas diminuta | ? | - |
? | |
| 3.1.8.1 | paraoxon + H2O | - |
Pseudomonas sp. | diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | paraoxon + H2O | - |
Agrobacterium tumefaciens | diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | paraoxon + H2O | - |
Homo sapiens | diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | paraoxon + H2O | - |
Brevundimonas diminuta | diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | parathion + H2O | - |
Pseudomonas sp. | diethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | parathion + H2O | - |
Agrobacterium tumefaciens | diethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | parathion + H2O | - |
Homo sapiens | diethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | parathion + H2O | - |
Brevundimonas diminuta | diethyl thiophosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | SP-CMP + H2O | SP-CMP is the toxic SP enantiomer of a cyclosarin surrogate in which the fluoride leaving group is replaced by a coumarin derivative | Homo sapiens | ? | - |
? | |
| 3.1.8.1 | SP-CMP + H2O | SP-CMP is the toxic SP enantiomer of a GF surrogate in which the fluoride leaving group is replaced by a coumarin derivative | Homo sapiens | ? | - |
? | |
| 3.1.8.1 | tabun + H2O | - |
Pseudomonas sp. | cyanophosphonic acid dimethylamide + ethanol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | OpdA | - |
Agrobacterium tumefaciens |
| 3.1.8.1 | paraoxonase | - |
Homo sapiens |
| 3.1.8.1 | parathion hydrolase | - |
Pseudomonas sp. |
| 3.1.8.1 | parathion hydrolase | - |
Brevundimonas diminuta |
| 3.1.8.1 | phosphotriesterase | - |
Agrobacterium tumefaciens |
| 3.1.8.1 | phosphotriesterase | - |
Brevundimonas diminuta |
| 3.1.8.1 | PON1 | - |
Homo sapiens |
| 3.1.8.1 | PTE | - |
Brevundimonas diminuta |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 25 | - |
assay at | Homo sapiens |
| 3.1.8.1 | 25 | 37 | assay at | Pseudomonas sp. |
| 3.1.8.1 | 25 | 37 | assay at | Brevundimonas diminuta |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 8 | - |
assay at | Pseudomonas sp. |
| 3.1.8.1 | 8 | - |
assay at | Homo sapiens |
| 3.1.8.1 | 8 | - |
assay at | Brevundimonas diminuta |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | additional information | protective ratio in mice pretreated i.v. with catalytic scavengers and challenged i.v. with tabun, paraoxon, O,O-diethyl fluorophosphate, and the cyclosarin surrogate, Sp-CMP, pH 8.0, 25°C or 38°C | Pseudomonas sp. |
| 3.1.8.1 | physiological function | evaluation of protective effect of the enzyme in plasma of rats against in vitro challenge of intoxication with parathion or paraoxon in rats, effectiveness of the enzyme as bioscavenger in vivo, overview | Pseudomonas sp. |
| 3.1.8.1 | physiological function | evaluation of protective effect of the enzyme in plasma of rats against in vitro challenge of intoxication with parathion or paraoxon in rats, effectiveness of the enzyme as bioscavenger in vivo, overview | Agrobacterium tumefaciens |
| 3.1.8.1 | physiological function | evaluation of protective effect of the enzyme in plasma of rats against in vitro challenge of intoxication with parathion or paraoxon in rats, effectiveness of the enzyme as bioscavenger in vivo, overview | Homo sapiens |
| 3.1.8.1 | physiological function | evaluation of protective effect of the enzyme in plasma of rats against in vitro challenge of intoxication with parathion or paraoxon in rats, effectiveness of the enzyme as bioscavenger in vivo, overview | Brevundimonas diminuta |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | 0.283 | - |
SP-CMP | pH 8.0, 25°C, enzyme administered in mice in vivo | Homo sapiens | |
| 3.1.8.1 | 0.367 | - |
paraoxon | pH 8.0, 25°C, enzyme administered in rats in vivo | Homo sapiens | |
| 3.1.8.1 | 0.367 | - |
parathion | pH 8.0, 25°C, enzyme administered in rats in vivo | Homo sapiens | |
| 3.1.8.1 | 0.767 | - |
Tabun | pH 8.0, 25°C, enzyme administered in mice in vivo | Pseudomonas sp. | |
| 3.1.8.1 | 0.867 | - |
O,O-diethyl fluorophosphate | pH 8.0, 25°C, enzyme administered in mice in vivo | Pseudomonas sp. | |
| 3.1.8.1 | 1.1 | - |
parathion | pH 8.0, 25°C, enzyme administered in rats in vivo | Agrobacterium tumefaciens | |
| 3.1.8.1 | 1.1 | - |
dichlorvos | pH 8.0, 37°C, enzyme administered in monkeys in vivo | Agrobacterium tumefaciens | |
| 3.1.8.1 | 23.33 | - |
paraoxon | pH 8.0, 38°C, enzyme administered in mice in vivo | Pseudomonas sp. | |
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