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Literature summary extracted from

  • Vilches, F.; Ravanal, M.C.; Bravo-Moraga, F.; Gonzalez-Nilo, D.; Eyzaguirre, J.
    Penicillium purpurogenum produces a novel endo-1,5-arabinanase, active on debranched arabinan, short arabinooligosaccharides and on the artificial substrate p-nitrophenyl arabinofuranoside (2018), Carbohydr. Res., 455, 106-113 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99 expression in Pichia pastoris Talaromyces purpureogenus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.99 EDTA inhibits slightly Talaromyces purpureogenus
3.2.1.99 EGTA inhibits slightly Talaromyces purpureogenus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.99 0.26
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.5, 37°C Talaromyces purpureogenus
3.2.1.99 0.47
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.5, 37°C Talaromyces purpureogenus
3.2.1.99 5.37
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 4.5, 37°C Talaromyces purpureogenus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.99 additional information the addition of Ca2+ has no effect Talaromyces purpureogenus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 33478
-
 calculated from sequence Talaromyces purpureogenus
3.2.1.99 38000
-
 SDS-PAGE Talaromyces purpureogenus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Talaromyces purpureogenus A0A2H4RAB6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99
-
 Talaromyces purpureogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.99 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + beta-D-glucopyranose
-
 ?
3.2.1.99 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.99 debranched arabinan + H2O
-
 Talaromyces purpureogenus arabinose + arabinobiose + arabinotriose
-
 ?
3.2.1.99 additional information no activity with birchwood xylan, beechwood xylan, citric pectin and arabinogalactan Talaromyces purpureogenus ?
-
 ?
3.2.1.99 sugar beet arabinan + H2O
-
 Talaromyces purpureogenus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.99 ? x * 38000, SDS-PAGE Talaromyces purpureogenus
3.2.1.99 ? x * 33478, calculated from sequence Talaromyces purpureogenus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 Abn1
-
 Talaromyces purpureogenus
3.2.1.99 endo-1,5-arabinanase
-
 Talaromyces purpureogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 30 35
-
 Talaromyces purpureogenus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.99 15 40 15°C: about 45% of maximal activity, 40°C: about 70% of maximal activity Talaromyces purpureogenus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.99 40
-
 good stability up to 40°C for 1 h Talaromyces purpureogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 4.5
-
-
 Talaromyces purpureogenus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.99 3.5 5 pH 3.5: about 45% of maximal activity, pH 5.0: about 50% of maximal activity Talaromyces purpureogenus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.99 4
-
 most stable at pH 4 Talaromyces purpureogenus