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Literature summary extracted from
- Investigation of debranching pattern of a thermostable isoamylase and its application for the production of resistant starch (2017), Carbohydr. Res., 446-447, 93-100 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.68 | expressed in Escherichia coli BL21(DE3) cells | Lederbergia lentus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.68 | starch + H2O | Lederbergia lentus | the enzyme has the ability to attack side chains composed of 1-3 glucose residues | amylose | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.68 | Lederbergia lentus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.68 | ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration | Lederbergia lentus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.68 | glycogen + H2O | - |
Lederbergia lentus | ? | - |
? | |
| 3.2.1.68 | maize amylopectin + H2O | isoamylase hydrolyses both inner and outer branching linkages of amylopectin simultaneously | Lederbergia lentus | ? | - |
? | |
| 3.2.1.68 | maize starch + H2O | - |
Lederbergia lentus | ? | - |
? | |
| 3.2.1.68 | additional information | no activity with amylose | Lederbergia lentus | ? | - |
? | |
| 3.2.1.68 | potato starch + H2O | - |
Lederbergia lentus | ? | - |
? | |
| 3.2.1.68 | soluble starch + H2O | - |
Lederbergia lentus | ? | - |
? | |
| 3.2.1.68 | starch + H2O | the enzyme has the ability to attack side chains composed of 1-3 glucose residues | Lederbergia lentus | amylose | - |
? | |
| 3.2.1.68 | waxy rice starch + H2O | - |
Lederbergia lentus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.68 | monomer | 1 * 108000, SDS-PAGE | Lederbergia lentus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.68 | glycogen-6-glucanohydrolase | - |
Lederbergia lentus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 70 | - |
- |
Lederbergia lentus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 6 | - |
- |
Lederbergia lentus |
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Release 2023.1 (January 2023)
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