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Literature summary extracted from

  • Belaz, S.; Rattier, T.; Lafite, P.; Moreau, P.; Routier, F.H.; Robert-Gangneux, F.; Gangneux, J.P.; Daniellou, R.
    Identification, biochemical characterization, and in-vivo expression of the intracellular invertase BfrA from the pathogenic parasite Leishmania major (2015), Carbohydr. Res., 415, 31-38 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.26 pharmacology putative target for antileishmanian drug development Leishmania donovani
3.2.1.26 pharmacology putative target for antileishmanian drug development Leishmania major
3.2.1.26 pharmacology putative target for antileishmanian drug development Leishmania braziliensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 heterologous overexpression in both Escherichia coli and Leishmania tarentolae Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 0.9
-
 inulin 37°C, pH 7.5 Leishmania major
3.2.1.26 141
-
 raffinose 37°C, pH 7.5 Leishmania major
3.2.1.26 152
-
 sucrose 37°C, pH 7.5 Leishmania major
3.2.1.26 168
-
 stachyose 37°C, pH 7.5 Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Leishmania braziliensis A4HCW0
-
-
3.2.1.26 Leishmania donovani
-
-
-
3.2.1.26 Leishmania major Q4QB75
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26
-
 Leishmania major

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 amastigote expressed to a higher extent in promastigotes than in amastigotes Leishmania donovani
-
3.2.1.26 amastigote expressed to a higher extent in promastigotes than in amastigotes Leishmania major
-
3.2.1.26 amastigote expressed to a higher extent in promastigotes than in amastigotes Leishmania braziliensis
-
3.2.1.26 promastigote expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase Leishmania donovani
-
3.2.1.26 promastigote expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase Leishmania major
-
3.2.1.26 promastigote expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase Leishmania braziliensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 1-kestose + H2O 5.6% compared to the activity with sucrose Leishmania major D-fructose + ?
-
 ?
3.2.1.26 inulin + H2O 10.9% compared to the activity with sucrose Leishmania major D-fructose + D-glucose + fructooligosaccharides
-
 ?
3.2.1.26 additional information no hydrolysis when using 4-nitrophenyl-alpha-D-glucopyranose as substrate Leishmania major ?
-
 ?
3.2.1.26 raffinose + H2O 109% compared to the activity with sucrose Leishmania major beta-D-fructose + alpha-D-melibiose
-
 ?
3.2.1.26 stachyose + H2O 297% compared to the activity with sucrose Leishmania major D-fructose + alpha-D-Gal-(1->6)-alpha-D-Gal-(1->6)-alpha-D-Gal
-
 ?
3.2.1.26 sucrose
-
 Leishmania major beta-D-fructose + alpha-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 BfrA
-
 Leishmania donovani
3.2.1.26 BfrA
-
 Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 40
-
-
 Leishmania major

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.26 20 50 20°C: about 60% of maximal activity, 50°C: about 45% of maximal activity Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 4.2
-
 inulin 37°C, pH 7.5 Leishmania major
3.2.1.26 114
-
 sucrose 37°C, pH 7.5 Leishmania major
3.2.1.26 118
-
 raffinose 37°C, pH 7.5 Leishmania major
3.2.1.26 409
-
 stachyose 37°C, pH 7.5 Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 6
-
-
 Leishmania major

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 5 7.9 pH 5.0: about 75% of maximal activity, pH 7.0: about 75% of maximal activity Leishmania major

Expression

EC Number Organism Comment Expression
3.2.1.26 Leishmania major expressed to a higher extent in promastigotes than in amastigotes up
3.2.1.26 Leishmania braziliensis expressed to a higher extent in promastigotes than in amastigotes up

General Information

EC Number General Information Comment Organism
3.2.1.26 physiological function the enzyme could be important for the development and growth of the parasite in the sandfly gut Leishmania donovani
3.2.1.26 physiological function the enzyme could be important for the development and growth of the parasite in the sandfly gut Leishmania major
3.2.1.26 physiological function the enzyme could be important for the development and growth of the parasite in the sandfly gut Leishmania braziliensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.26 0.75
-
 sucrose 37°C, pH 7.5 Leishmania major
3.2.1.26 0.86
-
 raffinose 37°C, pH 7.5 Leishmania major
3.2.1.26 2.4
-
 stachyose 37°C, pH 7.5 Leishmania major
3.2.1.26 4.6
-
 inulin 37°C, pH 7.5 Leishmania major