EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.15 | L-histidinol | about 5% activation at 10 mM and above. Cg0911 is positively feedback regulated by L-histidinol | Corynebacterium glutamicum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.15 | expression in Escherichia coli | Corynebacterium glutamicum |
3.1.3.15 | gene cg0911 or cgl0800, sequence comparisons | Corynebacterium glutamicum |
3.1.3.15 | gene hisN or cg0910, sequence comparisons | Corynebacterium glutamicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.15 | additional information | generation of a DELTAhisN single mutant, a DELTAhisN DELTAcg0911 double mutant, and a quintuple mutant, lacking hisN and all its paralogues, phenotypes, overview. No further reduction of growth of the DELTAhisN DELTAcg0911 double or the DELTAhisN DELTAcg0911 DELTAimpA DELTAsuhB DELTAcysQ quintuple mutant as compared to the DELTAhisN single mutant. Supplementation with L-histidine results in the same growth of all mutants. Expression of impA, suhB or cysQ does not improve the growth of the DELTAhisN strain on minimal medium. Beside the complementation by hisN itself, a complementation of the DELTAhisN growth defect is only observed with cg0911. Although the complementation assay clearly demonstrates HolPase activity of the cg0911 gene product in vivo if expressed on a multiple copy plasmid, this activity does not account for L-histidine biosynthesis in a measurable degree if present in single copy under control of the native promotor | Corynebacterium glutamicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.15 | EDTA | 10 mM, complete loss of activity; 10 mM, complete loss of activity | Corynebacterium glutamicum | |
3.1.3.15 | additional information | no inhibitory effect is observed by L-histidine at up to 60 mM L-histidine, by phosphate at up to 0.25 mM, or by L-histidinol at 20 mM; no inhibitory effect of L-histidine is observed with concentrations up to 60 mM L-histidine | Corynebacterium glutamicum | |
3.1.3.15 | phosphate | 40% inhibition at 0.375 mM | Corynebacterium glutamicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.15 | 0.0236 | - |
L-histidinol phosphate | pH 7.4, 30°C, Hill coefficient 1.47 | Corynebacterium glutamicum | |
3.1.3.15 | 0.638 | - |
L-histidinol phosphate | pH 7.4, 30°C, Hill coefficient 1.47 | Corynebacterium glutamicum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.15 | Co2+ | 20% of the activity with Mg2+ | Corynebacterium glutamicum | |
3.1.3.15 | Co2+ | 20% activation compared to Mg2+ at 5 mM | Corynebacterium glutamicum | |
3.1.3.15 | Co2+ | 47% of the activity with Mg2+ | Corynebacterium glutamicum | |
3.1.3.15 | Co2+ | 50% activation compared to Mg2+ at 5 mM | Corynebacterium glutamicum | |
3.1.3.15 | Mg2+ | activates, best divalent cation | Corynebacterium glutamicum | |
3.1.3.15 | Mg2+ | assay in presence of 5 mM, Km value 0.65 mM, Hill coefficient 2.44 | Corynebacterium glutamicum | |
3.1.3.15 | Mn2+ | 11% of the activity with Mg2+ | Corynebacterium glutamicum | |
3.1.3.15 | Mn2+ | 78% of the activity with Mg2+ | Corynebacterium glutamicum | |
3.1.3.15 | Mn2+ | 10% activation compared to Mg2+ at 5 mM | Corynebacterium glutamicum | |
3.1.3.15 | Mn2+ | 75-80% activation compared to Mg2+ at 5 mM | Corynebacterium glutamicum | |
3.1.3.15 | additional information | divalent cation is required | Corynebacterium glutamicum | |
3.1.3.15 | additional information | no activating effect by Zn2+, Cu2+, Ca2+, Mi2+, and Fe2+ at 5 mM | Corynebacterium glutamicum | |
3.1.3.15 | additional information | no activating effect on activity by Zn2+, Cu2+, Ca2+, Mi2+, and Fe2+ at 5 mM | Corynebacterium glutamicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.15 | L-histidinol phosphate + H2O | Corynebacterium glutamicum | - |
L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | Corynebacterium glutamicum LMG 3730 | - |
L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | Corynebacterium glutamicum ATCC 13032 | - |
L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | Corynebacterium glutamicum JCM 1318 | - |
L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | Corynebacterium glutamicum NCIMB 10025 | - |
L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | Corynebacterium glutamicum DSM 20300 | - |
L-histidinol + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.15 | Corynebacterium glutamicum | - |
- |
- |
3.1.3.15 | Corynebacterium glutamicum | Q8NS79 | - |
- |
3.1.3.15 | Corynebacterium glutamicum | Q8NS80 | - |
- |
3.1.3.15 | Corynebacterium glutamicum ATCC 13032 | Q8NS79 | - |
- |
3.1.3.15 | Corynebacterium glutamicum ATCC 13032 | Q8NS80 | - |
- |
3.1.3.15 | Corynebacterium glutamicum DSM 20300 | - |
- |
- |
3.1.3.15 | Corynebacterium glutamicum DSM 20300 | Q8NS79 | - |
- |
3.1.3.15 | Corynebacterium glutamicum DSM 20300 | Q8NS80 | - |
- |
3.1.3.15 | Corynebacterium glutamicum JCM 1318 | Q8NS79 | - |
- |
3.1.3.15 | Corynebacterium glutamicum JCM 1318 | Q8NS80 | - |
- |
3.1.3.15 | Corynebacterium glutamicum LMG 3730 | Q8NS79 | - |
- |
3.1.3.15 | Corynebacterium glutamicum LMG 3730 | Q8NS80 | - |
- |
3.1.3.15 | Corynebacterium glutamicum NCIMB 10025 | Q8NS79 | - |
- |
3.1.3.15 | Corynebacterium glutamicum NCIMB 10025 | Q8NS80 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.15 | recombinant protein | Corynebacterium glutamicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.15 | L-histidinol phosphate + H2O | - |
Corynebacterium glutamicum | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | low activity | Corynebacterium glutamicum | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | - |
Corynebacterium glutamicum LMG 3730 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | low activity | Corynebacterium glutamicum LMG 3730 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | - |
Corynebacterium glutamicum ATCC 13032 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | low activity | Corynebacterium glutamicum ATCC 13032 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | - |
Corynebacterium glutamicum JCM 1318 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | low activity | Corynebacterium glutamicum JCM 1318 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | - |
Corynebacterium glutamicum NCIMB 10025 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | low activity | Corynebacterium glutamicum NCIMB 10025 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | - |
Corynebacterium glutamicum DSM 20300 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | L-histidinol phosphate + H2O | low activity | Corynebacterium glutamicum DSM 20300 | L-histidinol + phosphate | - |
? | |
3.1.3.15 | additional information | no substrate: 4-nitrophenyl phosphate | Corynebacterium glutamicum | ? | - |
? | |
3.1.3.15 | additional information | no substrate: 4-nitrophenyl phosphate | Corynebacterium glutamicum DSM 20300 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.15 | cg0910 | - |
Corynebacterium glutamicum |
3.1.3.15 | Cg0911 | - |
Corynebacterium glutamicum |
3.1.3.15 | Cgl0800 | - |
Corynebacterium glutamicum |
3.1.3.15 | hisN | - |
Corynebacterium glutamicum |
3.1.3.15 | HolPase | - |
Corynebacterium glutamicum |
3.1.3.15 | IMPase-like HolPase | - |
Corynebacterium glutamicum |
3.1.3.15 | inositol monophosphatase-like HolPase | - |
Corynebacterium glutamicum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.15 | 30 | - |
- |
Corynebacterium glutamicum |
3.1.3.15 | 30 | - |
recombinant enzyme | Corynebacterium glutamicum |
3.1.3.15 | 35 | 40 | - |
Corynebacterium glutamicum |
3.1.3.15 | 35 | 40 | recombinant enzyme HisN | Corynebacterium glutamicum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.15 | 20 | 40 | maximal Cg0911 activity at 30°C and less than 5% of this activity remains at 40°C, profile overview | Corynebacterium glutamicum |
3.1.3.15 | 20 | 50 | maximal HisN activity at 35-40°C, no activity at 50°C, profile overview | Corynebacterium glutamicum |
3.1.3.15 | 40 | - |
less than 5% residual activity | Corynebacterium glutamicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.15 | 0.013 | - |
L-histidinol phosphate | pH 7.4, 30°C | Corynebacterium glutamicum | |
3.1.3.15 | 1.04 | - |
L-histidinol phosphate | pH 7.4, 30°C | Corynebacterium glutamicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.15 | 7.3 | 7.4 | recombinant enzyme | Corynebacterium glutamicum |
3.1.3.15 | 7.4 | - |
- |
Corynebacterium glutamicum |
3.1.3.15 | 8 | - |
- |
Corynebacterium glutamicum |
3.1.3.15 | 8 | - |
recombinant enzyme | Corynebacterium glutamicum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.15 | 6.5 | 9.5 | recombinant enzyme, activity range, profile overview | Corynebacterium glutamicum |
3.1.3.15 | 7 | 10 | recombinant enzyme, maximal Cg0911 activity is observed at around pH 8 and is only little reduced at pH 7.35, followed by a sharp loss in activity towards more acidic conditions. The drop in activity towards more alkaline conditions is less pronounced, the enzyme exhibits still 30% of its activity at around pH 10, profile overview | Corynebacterium glutamicum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.15 | evolution | distribution of Cg0911 orthologues within bacteria, sequences and structure motifs comparisons, phylogenetic analysis, overview | Corynebacterium glutamicum |
3.1.3.15 | evolution | distribution of HisN orthologues within bacteria, sequences and structure motifs comparisons, phylogenetic analysis, overview | Corynebacterium glutamicum |
3.1.3.15 | malfunction | deletion of hisN in Corynebacterium glutamicum results in pronounced L-histidine bradytrophy instead of complete auxotrophy. Growth of the DELTAhisN mutant is visible after several days of incubation on minimal medium plates without L-histidine. Addition of L-histidine abolishes the observed growth defect completely. Complementation of the DELTAhisN growth defect is observed with gene cg0911 | Corynebacterium glutamicum |
3.1.3.15 | additional information | importance of a highly conserved aspartate residue accompanied by several aromatic amino acid residues present in motif 5 for HolPase activity | Corynebacterium glutamicum |
3.1.3.15 | physiological function | gene cg0911 encodes an enzyme with HolPase activity, the hisN paralogue gene cg0911 can complement the growth defect of mutant DELTAhisN. Enzyme Cg0911 is positively feedback regulated by L-histidinol | Corynebacterium glutamicum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.15 | 0.002 | - |
L-histidinol phosphate | pH 7.4, 30°C, Hill coefficient 1.47 | Corynebacterium glutamicum | |
3.1.3.15 | 44.1 | - |
L-histidinol phosphate | pH 7.4, 30°C, Hill coefficient 1.47 | Corynebacterium glutamicum |