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Literature summary extracted from

  • Liu, S.; Ding, S.
    Replacement of carbohydrate binding modules improves acetyl xylan esterase activity and its synergistic hydrolysis of different substrates with xylanase (2016), BMC Biotechnol., 16, 73 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.72 additional information construction of fusion proteins with xylan-specific CBM4-2, CBM6, and CBM22-2 modules from Rhodothermus marinus Xyn10A, Clostridium thermocellum Xyn11A, and Clostridium thermocellum Xyn10B, respectively. Replacement of CBM1 with xylan-specific CBM4-2 significantly enhances AXE1 thermostability and catalytic activity against substrate 4-methylumbelliferyl acetate. Replacements with CBM6 and CBM22-2 are more effective in enzymatic release of acetic acid from destarched wheat bran, NaClO2-treated wheat straw, and water-insoluble wheat arabinoxylan compared to AXE1. Replacement with CBM6 and CBM22-2 also results in higher degree releases of reducing sugar and acetic acid from different substrates Volvariella volvacea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.72 0.079
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM6, pH 8.0, 50°C Volvariella volvacea
3.1.1.72 0.087
-
 4-methylumbelliferyl acetate wild-type lacking CBM1, 50°C, pH 8.0 Volvariella volvacea
3.1.1.72 0.087
-
 4-methylumbelliferyl acetate wild-type, 50°C, pH 8.0 Volvariella volvacea
3.1.1.72 0.094
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM22-2, pH 8.0, 50°C Volvariella volvacea
3.1.1.72 0.099
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM4-2, pH 8.0, 50°C Volvariella volvacea

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Volvariella volvacea Q09IZ4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-methylumbelliferyl acetate + H2O
-
 Volvariella volvacea 4-methylumbelliferone + acetate
-
 ?
3.1.1.72 avicel + H2O
-
 Volvariella volvacea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 Axe1
-
 Volvariella volvacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 50
-
-
 Volvariella volvacea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 55
-
 90 min, 3% residual activity for wild-type, 40% residual activity for mutant carrying a replacement of CBM1 with CBM4-2 Volvariella volvacea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.72 456
-
 4-methylumbelliferyl acetate wild-type, 50°C, pH 8.0 Volvariella volvacea
3.1.1.72 485
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM6, pH 8.0, 50°C Volvariella volvacea
3.1.1.72 485
-
 4-methylumbelliferyl acetate wild-type lacking CBM1, 50°C, pH 8.0 Volvariella volvacea
3.1.1.72 562
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM22-2, pH 8.0, 50°C Volvariella volvacea
3.1.1.72 897
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM4-2, pH 8.0, 50°C Volvariella volvacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 8
-
-
 Volvariella volvacea
3.1.1.72 8.5
-
 recombinant protein, replacement of CBM1 with CBM6 Volvariella volvacea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.72 5250
-
 4-methylumbelliferyl acetate wild-type, 50°C, pH 8.0 Volvariella volvacea
3.1.1.72 5590
-
 4-methylumbelliferyl acetate wild-type lacking CBM1, 50°C, pH 8.0 Volvariella volvacea
3.1.1.72 5970
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM22-2, pH 8.0, 50°C Volvariella volvacea
3.1.1.72 6170
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM6, pH 8.0, 50°C Volvariella volvacea
3.1.1.72 9090
-
 4-methylumbelliferyl acetate mutant carrying a replacement of CBM1 with CBM4-2, pH 8.0, 50°C Volvariella volvacea