BRENDA - Enzyme Database

The GH5 1,4-beta-mannanase from Bifidobacterium animalis subsp. lactis Bl-04 possesses a low-affinity mannan-binding module and highlights the diversity of mannanolytic enzymes

Morrill, J.; Kulcinskaja, E.; Sulewska, A.M.; Lahtinen, S.; Stalbrand, H.; Svensson, B.; Abou Hachem, M.; BMC Biochem. 16, 26 (2015) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.78
expressed in Escherichia coli BL21 (DE3) cells
Bifidobacterium animalis subsp. lactis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.78
Bifidobacterium animalis subsp. lactis
-
-
-
3.2.1.78
Bifidobacterium animalis subsp. lactis Bl-04
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.78
glycoprotein
-
Bifidobacterium animalis subsp. lactis
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.78
HisTrap column chromatography and Superdex G75 gel filtration
Bifidobacterium animalis subsp. lactis
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.78
4°C, 10 mM HEPES, 4 days, no loss of activity at pH 8.0, 75% loss of activity at pH 6.0
Bifidobacterium animalis subsp. lactis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.78
insoluble mannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis
mannotriose + mannotetraose + mannopentaose
-
-
-
?
3.2.1.78
insoluble mannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis Bl-04
mannotriose + mannotetraose + mannopentaose
-
-
-
?
3.2.1.78
konjac glucomannan + H2O
highest activity
750179
Bifidobacterium animalis subsp. lactis
?
-
-
-
?
3.2.1.78
konjac glucomannan + H2O
highest activity
750179
Bifidobacterium animalis subsp. lactis Bl-04
?
-
-
-
?
3.2.1.78
locust bean galactomannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis
mannotriose + mannotetraose + mannopentaose
-
-
-
?
3.2.1.78
locust bean galactomannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis Bl-04
mannotriose + mannotetraose + mannopentaose
-
-
-
?
3.2.1.78
low-viscosity locust bean galactomannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis
?
-
-
-
?
3.2.1.78
mannopentaose + H2O
-
750179
Bifidobacterium animalis subsp. lactis
mannobiose + mannotriose + mannotetraose
-
-
-
?
3.2.1.78
mannotetraose + H2O
-
750179
Bifidobacterium animalis subsp. lactis
?
-
-
-
?
3.2.1.78
mannotetraose + H2O
-
750179
Bifidobacterium animalis subsp. lactis Bl-04
?
-
-
-
?
3.2.1.78
additional information
the enzyme displays no activity towards mannotriose, Avicel and guar gum
750179
Bifidobacterium animalis subsp. lactis
?
-
-
-
?
3.2.1.78
additional information
the enzyme displays no activity towards mannotriose, Avicel and guar gum
750179
Bifidobacterium animalis subsp. lactis Bl-04
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.78
1,4-beta-mannanase
-
Bifidobacterium animalis subsp. lactis
3.2.1.78
Man5_8
-
Bifidobacterium animalis subsp. lactis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.78
55
-
-
Bifidobacterium animalis subsp. lactis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.78
55.9
-
unfolding temperature
Bifidobacterium animalis subsp. lactis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.78
97
-
Mannopentaose
at pH 6.0 and 37°C
Bifidobacterium animalis subsp. lactis
3.2.1.78
1828
-
low-viscosity locust bean galactomannan
at pH 6.0 and 37°C
Bifidobacterium animalis subsp. lactis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.78
6
-
-
Bifidobacterium animalis subsp. lactis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.78
expressed in Escherichia coli BL21 (DE3) cells
Bifidobacterium animalis subsp. lactis
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.78
glycoprotein
-
Bifidobacterium animalis subsp. lactis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.78
HisTrap column chromatography and Superdex G75 gel filtration
Bifidobacterium animalis subsp. lactis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.78
4°C, 10 mM HEPES, 4 days, no loss of activity at pH 8.0, 75% loss of activity at pH 6.0
Bifidobacterium animalis subsp. lactis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.78
insoluble mannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis
mannotriose + mannotetraose + mannopentaose
-
-
-
?
3.2.1.78
insoluble mannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis Bl-04
mannotriose + mannotetraose + mannopentaose
-
-
-
?
3.2.1.78
konjac glucomannan + H2O
highest activity
750179
Bifidobacterium animalis subsp. lactis
?
-
-
-
?
3.2.1.78
konjac glucomannan + H2O
highest activity
750179
Bifidobacterium animalis subsp. lactis Bl-04
?
-
-
-
?
3.2.1.78
locust bean galactomannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis
mannotriose + mannotetraose + mannopentaose
-
-
-
?
3.2.1.78
locust bean galactomannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis Bl-04
mannotriose + mannotetraose + mannopentaose
-
-
-
?
3.2.1.78
low-viscosity locust bean galactomannan + H2O
-
750179
Bifidobacterium animalis subsp. lactis
?
-
-
-
?
3.2.1.78
mannopentaose + H2O
-
750179
Bifidobacterium animalis subsp. lactis
mannobiose + mannotriose + mannotetraose
-
-
-
?
3.2.1.78
mannotetraose + H2O
-
750179
Bifidobacterium animalis subsp. lactis
?
-
-
-
?
3.2.1.78
mannotetraose + H2O
-
750179
Bifidobacterium animalis subsp. lactis Bl-04
?
-
-
-
?
3.2.1.78
additional information
the enzyme displays no activity towards mannotriose, Avicel and guar gum
750179
Bifidobacterium animalis subsp. lactis
?
-
-
-
?
3.2.1.78
additional information
the enzyme displays no activity towards mannotriose, Avicel and guar gum
750179
Bifidobacterium animalis subsp. lactis Bl-04
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.78
55
-
-
Bifidobacterium animalis subsp. lactis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.78
55.9
-
unfolding temperature
Bifidobacterium animalis subsp. lactis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.78
97
-
Mannopentaose
at pH 6.0 and 37°C
Bifidobacterium animalis subsp. lactis
3.2.1.78
1828
-
low-viscosity locust bean galactomannan
at pH 6.0 and 37°C
Bifidobacterium animalis subsp. lactis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.78
6
-
-
Bifidobacterium animalis subsp. lactis