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Literature summary extracted from
- Identification and characterization of a novel carboxylesterase (FpbH) that hydrolyzes aryloxyphenoxypropionate herbicides (2017), Biotechnol. Lett., 39, 553-560 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | environmental protection | the catalytic efficiencies (kcat/Km) of Fluazifop-P-butyl carboxylesterase (FpbH) for different AOPP herbicides are higher than those of Cyhalofop-butyl esterase (ChbH) from Pseudomonas azotoformans and Fenoxaprop-ethyl hydrolase (FeH) from Rhodococcus sp.. FpbH differs from previously reported AOPP herbicide carboxylesterases and might be a good candidate enzyme for biodegradation, especially when diclofop-methyl and/or haloxyfop-P-methyl are the dominant pollutants | Aquamicrobium sp. FPB-1 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | gene fpbH, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Aquamicrobium sp. FPB-1 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.1 | Ag+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 |  |
| 3.1.1.1 | Al3+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Ca2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Co2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Cr3+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Cu2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Fe2+ | inhibits at 5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Fe3+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Hg2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Li+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Mg2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Mn2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | additional information | poor effect by EDTA at 5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Ni2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Pb2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | Zn2+ | inhibits at 0.5-5 mM | Aquamicrobium sp. FPB-1 | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 0.00032 | - |
methyl 2-(4-[[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 0.00039 | - |
ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 0.00039 | - |
methyl 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 0.00042 | - |
ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 0.00043 | - |
butyl 2-(4-[[5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 0.00045 | - |
butyl 2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | butyl 2-(4-[[5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate + H2O | Aquamicrobium sp. FPB-1 | - |
fluazifop-P + butanol | - |
? | |
| 3.1.1.1 | butyl 2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoate + H2O | Aquamicrobium sp. FPB-1 | - |
cyhalofop + butanol | - |
? | |
| 3.1.1.1 | ethyl 2-[4-[(6-chloro-1,3-benzoxazol-2-yl)oxy]phenoxy]propanoate | Aquamicrobium sp. FPB-1 | - |
fenoxaprop-P + ethanol | - |
? | |
| 3.1.1.1 | ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate + H2O | Aquamicrobium sp. FPB-1 | - |
quizalofop-P + ethanol | - |
? | |
| 3.1.1.1 | methyl 2-(4-[[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate + H2O | Aquamicrobium sp. FPB-1 | - |
haloxyfop-P + methanol | - |
? | |
| 3.1.1.1 | methyl 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoate + H2O | Aquamicrobium sp. FPB-1 | - |
diclofop + methanol | - |
? | |
| 3.1.1.1 | additional information | Aquamicrobium sp. FPB-1 | the Aquamicrobium strain FPB-1 is capable of efficiently degrading AOPP herbicides | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Aquamicrobium sp. FPB-1 | A0A024CDT8 | isolated from a legume field in Jiangsu Province, China | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Aquamicrobium sp. FPB-1 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | butyl 2-(4-[[5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate + H2O | - |
Aquamicrobium sp. FPB-1 | fluazifop-P + butanol | - |
? | |
| 3.1.1.1 | butyl 2-(4-[[5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate + H2O | i.e. fluazifop-P-butyl | Aquamicrobium sp. FPB-1 | fluazifop-P + butanol | - |
? | |
| 3.1.1.1 | butyl 2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoate + H2O | - |
Aquamicrobium sp. FPB-1 | cyhalofop + butanol | - |
? | |
| 3.1.1.1 | butyl 2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoate + H2O | i.e. cyhalofop-butyl | Aquamicrobium sp. FPB-1 | cyhalofop + butanol | - |
? | |
| 3.1.1.1 | ethyl 2-[4-[(6-chloro-1,3-benzoxazol-2-yl)oxy]phenoxy]propanoate | - |
Aquamicrobium sp. FPB-1 | fenoxaprop-P + ethanol | - |
? | |
| 3.1.1.1 | ethyl 2-[4-[(6-chloro-1,3-benzoxazol-2-yl)oxy]phenoxy]propanoate | i.e. fenoxaprop-P-ethyl | Aquamicrobium sp. FPB-1 | fenoxaprop-P + ethanol | - |
? | |
| 3.1.1.1 | ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate + H2O | - |
Aquamicrobium sp. FPB-1 | quizalofop-P + ethanol | - |
? | |
| 3.1.1.1 | ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate + H2O | i.e. quizalofop-P-ethyl | Aquamicrobium sp. FPB-1 | quizalofop-P + ethanol | - |
? | |
| 3.1.1.1 | methyl 2-(4-[[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate + H2O | - |
Aquamicrobium sp. FPB-1 | haloxyfop-P + methanol | - |
? | |
| 3.1.1.1 | methyl 2-(4-[[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate + H2O | i.e. haloxyfop-P-methyl | Aquamicrobium sp. FPB-1 | haloxyfop-P + methanol | - |
? | |
| 3.1.1.1 | methyl 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoate + H2O | - |
Aquamicrobium sp. FPB-1 | diclofop + methanol | - |
? | |
| 3.1.1.1 | methyl 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoate + H2O | i.e. diclofop-methyl | Aquamicrobium sp. FPB-1 | diclofop + methanol | - |
? | |
| 3.1.1.1 | additional information | the Aquamicrobium strain FPB-1 is capable of efficiently degrading AOPP herbicides | Aquamicrobium sp. FPB-1 | ? | - |
? | |
| 3.1.1.1 | additional information | the enzyme hydrolyzes aryloxyphenoxypropionate herbicides, mass spectrometry analysis of metabolite generated during fluazifop-P-butyl degradation by strain FPB-1, overview | Aquamicrobium sp. FPB-1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | ? | x * 28000, about, recombinant His-tagged enzyme, SDS-PAGE and sequence calculation | Aquamicrobium sp. FPB-1 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | AOPP herbicide carboxylesterase | - |
Aquamicrobium sp. FPB-1 |
| 3.1.1.1 | Fluazifop-P-butyl carboxylesterase | - |
Aquamicrobium sp. FPB-1 |
| 3.1.1.1 | Fluazifop-P-butyl hydrolase | UniProt | Aquamicrobium sp. FPB-1 |
| 3.1.1.1 | FpbH | - |
Aquamicrobium sp. FPB-1 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 37 | - |
- |
Aquamicrobium sp. FPB-1 |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 10 | 60 | activity range, profile overview | Aquamicrobium sp. FPB-1 |
| 3.1.1.1 | 15 | 50 | over 50% of maximal activity within this range | Aquamicrobium sp. FPB-1 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 4.17 | - |
butyl 2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 4.46 | - |
butyl 2-(4-[[5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 5.01 | - |
ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 5.28 | - |
ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 5.92 | - |
methyl 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 6.31 | - |
methyl 2-(4-[[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 7 | - |
- |
Aquamicrobium sp. FPB-1 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 4 | 9.5 | activity range, profile overview | Aquamicrobium sp. FPB-1 |
| 3.1.1.1 | 5 | 8.5 | over 50% of maximal activity within this range | Aquamicrobium sp. FPB-1 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | additional information | enzyme FpbH contains the catalytic triad formed by Ser100, Asp213, and His239, proposed model of FpbH generated using an alpha/beta-hydrolase template from Rhodopseudomonas palustris strain CGA009, PDB ID 4psu | Aquamicrobium sp. FPB-1 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 9266.7 | - |
butyl 2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 10372.1 | - |
butyl 2-(4-[[5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 12571.4 | - |
ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 12846.2 | - |
ethyl 2-[4-[(6-chloroquinoxalin-2-yl)oxy]phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 15179.5 | - |
methyl 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
| 3.1.1.1 | 19718.8 | - |
methyl 2-(4-[[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy]phenoxy)propanoate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aquamicrobium sp. FPB-1 | |
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