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Literature summary extracted from

  • Yu, S.; Li, Z.; Wang, Y.; Chen, W.; Fu, L.; Tang, W.; Chen, C.; Liu, Y.; Zhang, X.; Ma, L.
    High-level expression and characterization of a thermophilic beta-mannanase from Aspergillus niger in Pichia pastoris (2015), Biotechnol. Lett., 37, 1853-1859 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expressed in Pichia pastoris strain GS115 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Aspergillus niger A2QKT4
-
-
3.2.1.78 Aspergillus niger CBS 513.88 A2QKT4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
 Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 Ni-NTA resin column chromatography Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 guar gum + H2O
-
 Aspergillus niger mannobiose + mannotriose + mannose
-
 ?
3.2.1.78 guar gum + H2O
-
 Aspergillus niger CBS 513.88 mannobiose + mannotriose + mannose
-
 ?
3.2.1.78 konjaku flour + H2O
-
 Aspergillus niger mannobiose + mannotriose + mannose
-
 ?
3.2.1.78 konjaku flour + H2O
-
 Aspergillus niger CBS 513.88 mannobiose + mannotriose + mannose
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Aspergillus niger mannotriose + ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Aspergillus niger CBS 513.88 mannotriose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 45000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 Beta-mannanase
-
 Aspergillus niger
3.2.1.78 Man
-
 Aspergillus niger
3.2.1.78 MAN-P
-
 Aspergillus niger
3.2.1.78 mannan endo-1,4-beta-mannosidase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 80
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 50 85 the enzyme retains 60% activity at 80°C for 2 h and almost full enzyme activity remains after 4 h at 50°C. The enzyme retains 52 % enzyme activity at 85°C after 15 min Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 4.5
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 1.5 11 the enzyme is stable over the pH range 1.5-11.0. The enzyme retains 84 % of its activity at 50°C for 12 h in the pH range 3.5-9.0 Aspergillus niger