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Literature summary extracted from

  • Rabinovich, M.L.; Melnik, M.S.; Herner, M.L.; Voznyi, Y.V.; Vasilchenko, L.G.
    Predominant nonproductive substrate binding by fungal cellobiohydrolase I and implications for activity improvement (2019), Biotechnol. J., 14, e1700712 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 cellobiose
-
 Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 0.000074
-
 4-methylumbelliferyl beta-cellopentaoside at pH 5.0 and 10°C Trichoderma reesei
3.2.1.176 0.00015
-
 4-methylumbelliferyl beta-cellotetraoside at pH 5.0 and 10°C Trichoderma reesei
3.2.1.176 0.004
-
 4-trifluoromethylumbelliferyl beta-cellotrioside at pH 5.0 and 10°C Trichoderma reesei
3.2.1.176 0.006
-
 4-methylumbelliferyl beta-cellobioside at pH 5.0 and 10°C Trichoderma reesei
3.2.1.176 0.0073
-
 4-methylumbelliferyl beta-cellotrioside at pH 5.0 and 10°C Trichoderma reesei
3.2.1.176 0.0125
-
 4-trifluoromethylumbelliferyl beta-cellobioside at pH 5.0 and 10°C Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.176 cellulose + H2O Trichoderma reesei
-
 cellobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-methylumbelliferyl beta-cellobioside + H2O
-
 Trichoderma reesei 4-methylumbelliferone + cellobiose
-
 ?
3.2.1.176 4-methylumbelliferyl beta-cellopentaoside + H2O
-
 Trichoderma reesei 4-methylumbelliferone + cellopentaose
-
 ?
3.2.1.176 4-methylumbelliferyl beta-cellotetraoside + H2O
-
 Trichoderma reesei 4-methylumbelliferone + cellotetraose
-
 ?
3.2.1.176 4-methylumbelliferyl beta-cellotrioside + H2O
-
 Trichoderma reesei 4-methylumbelliferone + cellotriose
-
 ?
3.2.1.176 4-trifluoromethylumbelliferyl beta-cellobioside + H2O
-
 Trichoderma reesei 4-trifluoromethylumbelliferone + cellobiose
-
 ?
3.2.1.176 4-trifluoromethylumbelliferyl beta-cellotrioside + H2O
-
 Trichoderma reesei 4-trifluoromethylumbelliferone + cellotriose
-
 ?
3.2.1.176 cellulose + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 CBHI
-
 Trichoderma reesei
3.2.1.176 Cel7A
-
 Trichoderma reesei
3.2.1.176 cellobiohydrolase I
-
 Trichoderma reesei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.176 0.015
-
 cellobiose pH and temperature not specified in the publication Trichoderma reesei