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Literature summary extracted from

  • Barth, M.; Honak, A.; Oeser, T.; Wei, R.; Belisario-Ferrari, M.R.; Then, J.; Schmidt, J.; Zimmermann, W.
    A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films (2016), Biotechnol. J., 11, 1082-1087 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.101 degradation a dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films. Since the enzymatic PET hydrolysis is inhibited by the degradation intermediate 4-[(2-hydroxyethoxy)carbonyl]benzoate, a dual enzyme system consisting of a polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 is employed for the hydrolysis of PET films at 60°C. HPLC analysis of the reaction products obtained after 24 h of hydrolysis shows an increased amount of soluble products with a lower proportion of 4-[(2-hydroxyethoxy)carbonyl]benzoate in the presence of the immobilized carboxylesterase TfCa. The results indicate a continuous hydrolysis of the inhibitory 4-[(2-hydroxyethoxy)carbonyl]benzoate by the immobilized carboxylesterase TfCa and demonstrate its advantage as a second biocatalyst in combination with a polyester hydrolase for an efficient degradation oft PET films Thermobifida fusca
3.1.1.101 environmental protection a dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films. Since the enzymatic PET hydrolysis is inhibited by the degradation intermediate 4-[(2-hydroxyethoxy)carbonyl]benzoate, a dual enzyme system consisting of a polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 is employed for the hydrolysis of PET films at 60°C. HPLC analysis of the reaction products obtained after 24 h of hydrolysis shows an increased amount of soluble products with a lower proportion of 4-[(2-hydroxyethoxy)carbonyl]benzoate in the presence of the immobilized carboxylesterase TfCa. The results indicate a continuous hydrolysis of the inhibitory 4-[(2-hydroxyethoxy)carbonyl]benzoate by the immobilized carboxylesterase TfCa and demonstrate its advantage as a second biocatalyst in combination with a polyester hydrolase for an efficient degradation oft PET films Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.101 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.101 Thermobifida fusca E5BBQ3
-
-
3.1.1.101 Thermobifida fusca KW3 E5BBQ3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.101 (ethylene terephthalate)n + H2O
-
 Thermobifida fusca (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 ?
3.1.1.101 (ethylene terephthalate)n + H2O
-
 Thermobifida fusca KW3 (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.101 TfCut2
-
 Thermobifida fusca