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Literature summary extracted from

  • Regel, E.K.; Weikert, T.; Niehues, A.; Moerschbacher, B.M.; Singh, R.
    Protein-engineering of chitosanase from Bacillus sp. MN to alter its substrate specificity (2018), Biotechnol. Bioeng., 115, 863-873 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.132 agriculture partially acetylated chitosan oligosaccharides have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities. A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products Bacillus sp. MN
3.2.1.132 food industry partially acetylated chitosan oligosaccharides have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities. A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products Bacillus sp. MN
3.2.1.132 pharmacology partially acetylated chitosan oligosaccharides have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities. A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products Bacillus sp. MN

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expression in Escherichia coli Bacillus sp. MN

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 E309R/N319E the mutant enzyme can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wild-type enzyme Bacillus sp. MN
3.2.1.132 N308V/E309R/N319E the mutant enzyme can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wild-type enzyme Bacillus sp. MN

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus sp. MN H9CXV8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O the mutant enzymes E309R/N319E and N308V/E309R/N319E can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wild-type enzyme Bacillus sp. MN ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 BspCsnMN
-
 Bacillus sp. MN