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Literature summary extracted from

  • Choi, K.Y.; Jung, E.O.; Jung, D.H.; Pandey, B.P.; Lee, N.; Yun, H.; Park, H.Y.; Kim, B.G.
    Novel iron-sulfur containing NADPH-reductase from Nocardia farcinica IFM10152 and fusion construction with CYP51 lanosterol demethylase (2012), Biotechnol. Bioeng., 109, 630-636 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.15.36 synthesis construction of an artificial self-sufficient cytochrome P450 monooxygenase by fusion of NADPH-P450 reductase FprD, CYP51, and iron-sulfur containing FprD. CYP51-FprD fusion enzymes F1 and F2 in recombinant Escherichia coli catalyze demethylation of lanosterol more efficiently, with kcat/Km values about 35fold higher compared to those of CYP51 and FprD alone Nocardia farcinica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.36 0.0103
-
 lanosterol FprD fusion construct F1, pH 7.0, temperature not specified in the publication Nocardia farcinica
1.14.15.36 0.0122
-
 lanosterol wild-type, pH 7.0, temperature not specified in the publication Nocardia farcinica
1.14.15.36 0.0125
-
 lanosterol FprD fusion construct F2, pH 7.0, temperature not specified in the publication Nocardia farcinica

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.36 Nocardia farcinica Q5YWK5
-
-
1.14.15.36 Nocardia farcinica IFM 10152 Q5YWK5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.36 lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
-
 Nocardia farcinica 4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.15.36 lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
-
 Nocardia farcinica IFM 10152 4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.36 0.67
-
 lanosterol wild-type, pH 7.0, temperature not specified in the publication Nocardia farcinica
1.14.15.36 16.7
-
 lanosterol FprD fusion construct F1, pH 7.0, temperature not specified in the publication Nocardia farcinica
1.14.15.36 21.9
-
 lanosterol FprD fusion construct F2, pH 7.0, temperature not specified in the publication Nocardia farcinica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.15.36 50.5
-
 lanosterol wild-type, pH 7.0, temperature not specified in the publication Nocardia farcinica
1.14.15.36 1615
-
 lanosterol FprD fusion construct F1, pH 7.0, temperature not specified in the publication Nocardia farcinica
1.14.15.36 1763
-
 lanosterol FprD fusion construct F2, pH 7.0, temperature not specified in the publication Nocardia farcinica