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Literature summary extracted from

  • Suermeli, Y.; Ilgue, H.; Sanli-Mohamed, G.
    Improved activity of alpha-L-arabinofuranosidase from Geobacillus vulcani GS90 by directed evolution Investigation on thermal and alkaline stability (2019), Biotechnol. Appl. Biochem., 66, 101-107 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Escherichia coli BL21(lambdaDE3) cells Geobacillus vulcani

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.55 L307S the mutant shows almost 2.5fold increased activity and exhibits higher thermal stability but lower alkaline stability compared to the wild type enzyme Geobacillus vulcani
3.2.1.55 Q90H/L307S the mutant shows almost 2.5fold increased activity and exhibits higher thermal stability but lower alkaline stability compared to the wild type enzyme Geobacillus vulcani

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Geobacillus vulcani
-
-
-
3.2.1.55 Geobacillus vulcani GS90
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 His-tag nickel affinity column chromatography Geobacillus vulcani

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 72.066
-
 wild type enzyme, at pH 5.0 and 70°C Geobacillus vulcani
3.2.1.55 142.024
-
 mutant enzyme Q90H/L307S, at pH 5.0 and 70°C Geobacillus vulcani
3.2.1.55 160.991
-
 mutant enzyme L307S, at pH 5.0 and 70°C Geobacillus vulcani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus vulcani 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus vulcani GS90 4-nitrophenol + alpha-L-arabinofuranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 60000, SDS-PAGE Geobacillus vulcani

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 ABF
-
 Geobacillus vulcani
3.2.1.55 alpha-L-arabinofuranosidase
-
 Geobacillus vulcani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 70
-
-
 Geobacillus vulcani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 70 72 the wild type enzyme retains about 20% activity after 100 min at 70°C, about 20% activity after 40 min at 71°C, and less than 5% activity after 30 min at 72°C Geobacillus vulcani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5
-
-
 Geobacillus vulcani

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 5 9.6 the wild type enzyme retains about 20% activity after 100 min at pH 5.0 and 70°C and about 30% activity after 80 min at pH 9.6 and 70°C Geobacillus vulcani