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Literature summary extracted from

  • Mitsukawa, Y.; Hibi, M.; Matsutani, N.; Horinouchi, N.; Takahashi, S.; Ogawa, J.
    A novel nucleoside hydrolase from Lactobacillus buchneri LBK78 catalyzing hydrolysis of 2-O-methylribonucleosides (2016), Biosci. Biotechnol. Biochem., 80, 1568-1576 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.1 expression in Escherichia coli Lentilactobacillus buchneri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.1 0.04
-
 2'-O-methyluridine pH 7.0, 28°C Lentilactobacillus buchneri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.1 130000
-
 gel filtration Lentilactobacillus buchneri

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Lentilactobacillus buchneri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1 recombinant protein Lentilactobacillus buchneri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.1 0.671
-
 pH 7.0, 28°C Lentilactobacillus buchneri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 2'-O-methyladenosine + H2O
-
 Lentilactobacillus buchneri 2-O-methylribose + adenine
-
 ?
3.2.2.1 2'-O-methylcytidine + H2O
-
 Lentilactobacillus buchneri 2-O-methylribose + cytosine
-
 ?
3.2.2.1 2'-O-methylguanosine + H2O
-
 Lentilactobacillus buchneri 2-O-methylribose + guanidine
-
 ?
3.2.2.1 2'-O-methylinosine + H2O
-
 Lentilactobacillus buchneri 2-O-methylribose + hypoxanthine
-
 ?
3.2.2.1 2'-O-methyluridine + H2O
-
 Lentilactobacillus buchneri 2-O-methylribose + uracil
-
 ?
3.2.2.1 guanosine + H2O
-
 Lentilactobacillus buchneri D-ribose + guanine
-
 ?
3.2.2.1 additional information the enzyme prefers ribonucleosides and 2'-O-methylribonucleosides as its hydrolytic substrates, but reacts weakly with 2'-deoxyribonucleosides Lentilactobacillus buchneri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.1 tetramer 4 * 34000, SDS-PAGE Lentilactobacillus buchneri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.1 70
-
-
 Lentilactobacillus buchneri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.1 30 70 more than 80% of maximum activity Lentilactobacillus buchneri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.1 0.49
-
 2'-O-methyluridine pH 7.0, 28°C Lentilactobacillus buchneri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.1 3 10 1 h, more than 70% residual activity Lentilactobacillus buchneri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.2.1 12
-
 2'-O-methyluridine pH 7.0, 28°C Lentilactobacillus buchneri