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Literature summary extracted from

  • Nakayama, K.; Ohmori, T.; Ishikawa, S.; Iwata, N.; Seto, Y.; Kawahara, K.
    Expression of recombinant organophosphorus hydrolase in the original producer of the enzyme, Sphingobium fuliginis ATCC 27551 (2016), Biosci. Biotechnol. Biochem., 80, 1024-1026 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.8.1 gene opd, plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis is modified to be transferred back to this bacterium. The replication function of Sphingobium amiense plasmid is inserted at downstream of OPH gene, and Shingobium fuliginis is transformed with this plasmid, pTV118N-His-OPH-ORF4, the transformant is designated KGU0379. The transformant produces larger amount of active His-tagged OPH than Escherichia coli. Recombinant expression of plasmid pTV118N-His-OPH-ORF4 also in Sphingomonas paucimobilis strain KGU0001 (originally IAM 12576), the transformant is designated KGU0400. Recombinant expression in Escherichia coli resulting in strain KGU0255 Sphingobium fuliginis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.8.1 3-[fluoro(methyl)phosphoryl]oxy-2,2-dimethylbutane + H2O Sphingobium fuliginis i.e. soman ?
-
 ?
3.1.8.1 cyclohexylmethylphosphonofluoridate + H2O Sphingobium fuliginis i.e. cyclosarin ?
-
 ?
3.1.8.1 diethyl-paraoxon + H2O Sphingobium fuliginis
-
 diethyl phosphate + 4-nitrophenol
-
 ?
3.1.8.1 diethyl-parathion + H2O Sphingobium fuliginis
-
 diethyl thiophosphate + 4-nitrophenol
-
 ?
3.1.8.1 ethyl dimethylphosphoramidocyanidate + H2O Sphingobium fuliginis i.e. tabun ?
-
 ?
3.1.8.1 O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O Sphingobium fuliginis i.e. VX ?
-
 ?
3.1.8.1 O-isobutyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O Sphingobium fuliginis i.e. RVX, VR, or Russian VX ?
-
 ?
3.1.8.1 O-isopropyl methylphosphonofluoridate + H2O Sphingobium fuliginis i.e. sarin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.1 Sphingobium fuliginis K4I2M6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.1 recombinant His-tagged enzymes from Sphingobium fuliginis strain KGU0379 and Sphingomonas paucimobilis strain KGU0001 by nickel affinity chromatography Sphingobium fuliginis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.1 3-[fluoro(methyl)phosphoryl]oxy-2,2-dimethylbutane + H2O i.e. soman Sphingobium fuliginis ?
-
 ?
3.1.8.1 cyclohexylmethylphosphonofluoridate + H2O i.e. cyclosarin Sphingobium fuliginis ?
-
 ?
3.1.8.1 diethyl-paraoxon + H2O
-
 Sphingobium fuliginis diethyl phosphate + 4-nitrophenol
-
 ?
3.1.8.1 diethyl-parathion + H2O
-
 Sphingobium fuliginis diethyl thiophosphate + 4-nitrophenol
-
 ?
3.1.8.1 ethyl dimethylphosphoramidocyanidate + H2O i.e. tabun Sphingobium fuliginis ?
-
 ?
3.1.8.1 additional information reactions are performed with soluble recombinant enzyme in solution or immobilized recombinant enzyme in calcium alginate gel Sphingobium fuliginis ?
-
 ?
3.1.8.1 O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O i.e. VX Sphingobium fuliginis ?
-
 ?
3.1.8.1 O-isobutyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O i.e. RVX, VR, or Russian VX Sphingobium fuliginis ?
-
 ?
3.1.8.1 O-isopropyl methylphosphonofluoridate + H2O i.e. sarin Sphingobium fuliginis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 opd
-
 Sphingobium fuliginis
3.1.8.1 OPH
-
 Sphingobium fuliginis
3.1.8.1 organophosphorus hydrolase
-
 Sphingobium fuliginis
3.1.8.1 parathion hydrolase UniProt Sphingobium fuliginis