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Literature summary extracted from

  • Jiang, S.; Jiang, H.; Zhou, Y.; Jiang, S.; Zhang, G.
    High-level expression of beta-N-acetylglucosaminidase BsNagZ in Pichia pastoris to obtain GlcNAc (2019), Bioprocess Biosyst. Eng., 42, 611-619 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in Pichia pastoris strain X33 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 chitin + H2O Bacillus subtilis
-
 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitin + H2O Bacillus subtilis 168
-
 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitosan + H2O Bacillus subtilis
-
 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitosan + H2O Bacillus subtilis 168
-
 N-acetyl-beta-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bacillus subtilis P40406
-
-
3.2.1.52 Bacillus subtilis 168 P40406
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 His-Trap column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Bacillus subtilis 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Bacillus subtilis 168 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitin + H2O
-
 Bacillus subtilis N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitin + H2O
-
 Bacillus subtilis 168 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitosan + H2O
-
 Bacillus subtilis N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitosan + H2O
-
 Bacillus subtilis 168 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 colloidal chitin + H2O
-
 Bacillus subtilis N-acetyl-beta-D-glucosamine + ?
-
 ?
3.2.1.52 colloidal chitin + H2O
-
 Bacillus subtilis 168 N-acetyl-beta-D-glucosamine + ?
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Bacillus subtilis N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Bacillus subtilis 168 N-acetyl-beta-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 70000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 beta-N-acetylglucosaminidase
-
 Bacillus subtilis
3.2.1.52 NAGase
-
 Bacillus subtilis
3.2.1.52 NagZ
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 60
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 45 65 the enzyme maintains over 60% relative activity from 45 to 65°C Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 55 60 the enzyme keeps over 80% activity after pre-incubation at 55°C for 1 h and 60% residual activity after 10 min pre-incubation at 60°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 6
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 5.5 7 the enzyme remains over 60% relative activity from pH 5.5-7.0 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4.5 10 the enzyme keeps over 80% activity after pre-incubation at 4°C for 12 h from pH 4.5-10.0 Bacillus subtilis