Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rybak, M.; Kovalenko, O.; Kryklyvyi, I.; Tukalo, M.
    Cloning, expression and purification of D-Tyr-tRNATyr-deacylase from Thermus thermophilus (2015), Biopolym. Cell, 31, 179-186 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.96 cDNA encoding D-Tyr-tRNATyr-deacylase from Thermus thermophilus is cloned into pProEXHTb vector and successfully expressed in BL21(DE3)pLysS Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.96 32000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.96 D-tyrosyl-tRNATyr + H2O Thermus thermophilus
-
 D-tyrosine + tRNATyr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.96 Thermus thermophilus Q5SJQ9
-
-
3.1.1.96 Thermus thermophilus Q72K35
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.96
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.96 D-tyrosyl-tRNATyr + H2O
-
 Thermus thermophilus D-tyrosine + tRNATyr
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.96 dimer 2 * 16700, calculated from sequence Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.96 D-Tyr-tRNATyr-deacylase
-
 Thermus thermophilus