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Literature summary extracted from

  • Kozarski, M.; Kubacka, D.; Wojtczak, B.A.; Kasprzyk, R.; Baranowski, M.R.; Kowalska, J.
    7-Methylguanosine monophosphate analogues with 5-(1,2,3-triazoyl) moiety Synthesis and evaluation as the inhibitors of cNIIIB nucleotidase (2018), Bioorg. Med. Chem., 26, 191-199 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.5 expressed in Escherichia coli BL21(DE3) RIL cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 5'-deoxy-7-methyl-5'-(4-phosphono-1H-pyrazol-1-yl)guanosine moderately potent inhibitor Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mg2+ 5 mM used in assay conditions Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 HisTrap column chromatography and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 7-methylguanosine diphosphate + H2O preferred substrate Homo sapiens 7-methylguanosine monophosphate + phosphate
-
 ?
3.1.3.5 7-methylguanosine monophosphate + H2O preferred substrate Homo sapiens 7-methylguanosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 cNIIIB nucleotidase
-
 Homo sapiens
3.1.3.5 cytosolic 5'-nucleotidase
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.5 0.0878
-
 at pH 7.5 and 30°C Homo sapiens 5'-deoxy-7-methyl-5'-(4-phosphono-1H-pyrazol-1-yl)guanosine