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Literature summary extracted from

  • Kumar, D.; Yadav, S.; Yadava, S.; Yadav, K.D.S.
    An alkali tolerant alpha-L-rhamnosidase from Fusarium moniliforme MTCC-2088 used in de-rhamnosylation of natural glycosides (2019), Bioorg. Chem., 84, 24-31 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.40 food industry the purified enzyme has potential for enhancement of wine aroma Fusarium verticillioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 0.5
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 10.5 (0.2M sodium phosphate buffer), 50°C Fusarium verticillioides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.40 extracellular
-
 Fusarium verticillioides
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 36000
-
 SDS-PAGE Fusarium verticillioides

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Fusarium verticillioides
-
-
-
3.2.1.40 Fusarium verticillioides MTCC-2088
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Fusarium verticillioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Fusarium verticillioides 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Fusarium verticillioides MTCC-2088 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 hesperidin + H2O
-
 Fusarium verticillioides hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O
-
 Fusarium verticillioides MTCC-2088 hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Fusarium verticillioides beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Fusarium verticillioides MTCC-2088 beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Fusarium verticillioides isoquercitrin + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Fusarium verticillioides MTCC-2088 isoquercitrin + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 ? x * 36000, SDS-PAGE Fusarium verticillioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 50
-
-
 Fusarium verticillioides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 10
-
 1 h, 5% loss of activity Fusarium verticillioides
3.2.1.40 20
-
 1 h, 20% loss of activity Fusarium verticillioides
3.2.1.40 30
-
 1 h, 35% loss of activity Fusarium verticillioides
3.2.1.40 40
-
 1 h, 60% loss of activity Fusarium verticillioides
3.2.1.40 50
-
 1 h, 80% loss of activity Fusarium verticillioides
3.2.1.40 60
-
 1 h, 90% loss of activity Fusarium verticillioides
3.2.1.40 70
-
 1 h, complete loss of activity Fusarium verticillioides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.40 15.6
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 10.5 (0.2M sodium phosphate buffer), 50°C Fusarium verticillioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 9.5
-
 at 40°C Fusarium verticillioides
3.2.1.40 10.5
-
 at 60°C Fusarium verticillioides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 9 10 24 h, the enzyme is most stable between pH 9.0 and pH 10.0 Fusarium verticillioides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.40 31.2
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 10.5 (0.2M sodium phosphate buffer), 50°C Fusarium verticillioides